摘要
利用生物信息学手段鉴定了76个具有典型R结构的菠菜转录因子(Spinacia oleracea)MYB,其中包括72条R2R3-MYB基因(2R-MYB)和4条R1R2R3-MYB基因(3R-MYB)。通过生物信息学对菠菜MYB转录因子家族成员的理化性质、染色体定位、结构域序列保守性和系统进化关系进行了分析,结果表明:菠菜MYB家族有32个基因位于染色体正链,另外44个基因位于染色体反链;MYB的DNA结合域中的保守域主要位于两个R重复序列的第二和第三螺旋之间,结合域中每个R重复的第一和第二色氨酸之间的氨基酸序列相对不保守;根据菠菜、拟南芥及甜菜的MYB家族系统进化关系可以推测,菠菜MYB家族中56个成员可以按功能划分为4类,在菠菜的生长发育过程中可能起着重要的调节作用,其余成员中有7个MYB基因可能参与菠菜响应氮素浓度的氮素利用及生长发育进程。
In this study,76 transcription factor genes within MYB gene family were identified.According to their structural characteristics,these MYB genes were divided into two categories,i.e.R2R3-MYB and R1R2R3-MYB.Based on the Prot Param,Soft Berry,MEGA7.0 and other online softwares were applied for bioinformatics analyses,such as physical and chemical properties,subcellular localization,and system evolution.According to their MYB conserved domain numbers,76 spinach(Spinacia oleracea)MYB genes were identified and divided into two subgroups,2R-MYB(72)and R1R2R3-MYB(4).Chromosome mapping shows,spinach’s MYB family has 32 genes in the chromosome chain,the other 44 genes are located in the reverse chain of chromosomes.Conservative domain analysis shows that,the conserved domains in the MYB DNA binding domain are mainly located between the second and third spirals of the two R repeats while the amino acid sequences between the first and second tryptophan repeats of each R in the DNA binding domain are relatively unconservative.The phylogenetic analysis indicated that spinach,Arabidopsis thaliana and beet MYB transcription factor proteins can be divided into thirty-five clusters.It was speculated that the 56 members,which can be functionally divided into 4 groups,of the spinach MYB family,plays an important regulatory role in the growth and development of spinach.The seven MYB genes from other members may be involved in the process of nitrogen utilization and development in response to nitrogen concentration in spinach.
作者
王晓珊
葛晨辉
康亚妮
王全华
徐晨曦
WANG Xiaoshan;GE Chenhui;KANG Yani;WANG Quanhua;XU Chenxi(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Development Center of Plant Germplasm Resources,Shanghai Normal University,Shanghai 200234,China)
出处
《上海师范大学学报（自然科学版）》
2020年第6期677-691,共15页
Journal of Shanghai Normal University(Natural Sciences)
基金
国家自然科学基金青年基金(31601769)
上海市科研创新行动计划(16391901000)
上海植物种质资源工程技术研究中心项目(17DZ2252700)。
