摘要
目的建立同时测定生活饮用水中8种N-亚硝胺类化合物的气相色谱-串联质谱检测方法。方法取1.0 L水样,加入20 g氯化钠,2.0 g碳酸氢钠,调节水样pH值后,以5.0 ml/min^10.0 ml/min流速过椰壳活性炭固相萃取柱富集、二氯甲烷洗脱收集后,以D6-N-亚硝基二甲胺和D14-N-亚硝基-二正丙基亚硝胺为内标,采用Agilent VF-WAXms (30 m×250μm,1μm)色谱柱分离,气相色谱-串联质谱仪检测。结果在5 ng/L^400 ng/L内,方法线性关系良好(r> 0.999),最低检出限为0.65 ng/L^7.81 ng/L,定量限为2.15 ng/L^26.04 ng/L;在加标水平为10.0 ng/L,50.0 ng/L时,平均回收率为68.72%~125.39%,相对标准偏差为2.64%~13.45%。结论该方法快速、灵敏、准确度高,适用于饮用水中N-亚硝胺类化合物的测定。
Objective To establish a method for the simultaneous determination of 8 N-nitrosamines in drinking water by gas chromatography-tandem mass spectrometry(GC-MS/MS).Methods A total of 20.0 g sodium chloride and 2.0g sodium bicarbonate were added to 1.0L water sample to adjust its pH value.The target analytes in water were concentrated with coconut charcoal cartridges at a speed of 5.0 ml/min-10.0 ml/min,and then eluted with methylene chloride,and finally were separated with Agilent VF-WAXms column(30m×250μm,1μm)and detected by gas chromatography-tandem mass spectrometry.D6-N-nitrosodimethylamine and D14-N-nitrosodi-n-propylamine were used as internal standards.Results The linear ranges of the method were within 5 ng/L-400 ng/L,with the coeffcients of correlation greater than 0.999.The detection limits of the method were within 0.65 ng/L-7.81 ng/L,while the quantification limits were within 2.15 ng/L-26.04 ng/L.The average recoveries of the method spiked at 10 ng/L,50.0 ng/L ranged from 68.72%to 125.39%,with the relative standard deviation within 2.64%-13.45%(n=6).Conclusion This method is quick,sensitive and accurate and it can be used for determination of 8 N-nitrosamines in drinking water.
作者
张力群
金铨
唐涵
何华丽
刘少颖
黄希汇
ZHANG Li-qun;JIN Quan;TANG Han;HE Hua-li;LIU Shao-ying;HUANG Xi-hui(Hangzhou Municipal Center for Disease Control and Prevention,Hangzhou,Zhejiang 310021,China;不详)
出处
《中国卫生检验杂志》
CAS
2020年第24期2955-2958,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江省医药卫生科技计划(2019PY068)
杭州市医药卫生科技项目(A20200150)。
关键词
饮用水
N-亚硝胺
消毒副产物
气相色谱-串联质谱法
Drinking water
N-nitrosam ines
Disinfection byproducts
Gas chromatography-tandem mass spectrometry
