摘要
目的探讨骨化三醇对糖尿病肾病(DN)大鼠尿液中信号蛋白Smad1水平的影响及尿蛋白改变情况。方法采用链尿佐菌素(STZ)腹腔注射的方法构建糖尿病肾病大鼠动物模型,将造模成功的大鼠随机分为骨化三醇组(T组)10只、DN组(D组)10只,并设置健康对照组(N组)10只。T组大鼠给予0.03μg/(kg·d)的骨化三醇灌胃;D组与N组给予等量花生油灌胃。药物干预12周后检测各组大鼠尿液Smad1水平、24h尿蛋白定量及一般生化指标。结果T组和D组Smad1水平和24h尿蛋白定量高于N组,差异有统计学意义(P<0.05);T组尿液Smad1水平和24h尿蛋白定量低于D组,差异有统计学意义(P<0.05)。尿液Smad1水平与24h尿蛋白量呈正相关(r=0.671,P<0.05)。结论骨化三醇可能是通过调节信号蛋白Smad1水平,从而改善糖尿病肾病大鼠蛋白尿的产生。
Objective To investigate the effect of calcitriol on the level of signal protein Smad1 in the urine of diabetic nephropathy(DN)rats and the changes in urine protein.Methods The rat model of diabetic nephropathy was constructed by intraperitoneal injection of streptozotocin(STZ).The rats that were successfully modeled were randomly divided into calcitriol group(T group),10 rats,DN group(D group)10 animals,and a healthy control group(N group)10 animals.The rats in group T were given 0.03μg/(kg·d)calcitriol by gavage;group D and group N were given peanut oil by gavage.After 12 weeks of drug intervention,the levels of urine Smad1,24h urine protein quantitative and general biochemical indicators of each group were detected.Results The levels of Smad1 and 24h urine protein in group T and D were higher than those in group N,the difference was statistically significant(P<0.05);the level of urine Smad1 and 24h urine protein in group T were lower than those in group D,the difference was statistically significant(P<0.05).The level of urine Smad1 was positively correlated with 24h urine protein(r=0.671,P<0.05).Conclusion Calcitriol may improve the production of proteinuria in rats with diabetic nephropathy by regulating the level of signal protein Smad1.
作者
兰凯
张光伟
刘树义
张旭东
田甜
LAN Kai;ZHANG Guang-wei;LIU Shu-yi;ZHANG Xu-dong;TIAN Tian(Clinical School,Xi'an Medical University,Xi'an 710021,Shaanxi,China)
出处
《医学信息》
2021年第1期75-77,共3页
Journal of Medical Information
基金
陕西省教育厅科研计划项目(编号:19JK0762)。
