摘要
为了从全基因组水平筛选猪蛔虫微卫星分子标记,通过PCR扩增和毛细管电泳技术进行基因分型。结果显示,筛选获得了25个能稳定扩增并具有多态性的微卫星位点。这些位点的等位基因数(N A)为2~25个,观测杂合度(H O)和期望杂合度(H E)分别为0~1和0.042~0.945,多态信息含量(PIC)为0.040~0.921,其中20对显示中、高度多态性,无效等位基因频率(F)和近交系数(F IS)变化区间为0~0.1971和-0.1985~1,显示杂合子缺失。25对微卫星分子标记中有15对符合哈迪-温伯格平衡,10对或多或少的偏离平衡。且这些位点均能在人蛔虫中稳定扩增。本试验开发的微卫星标记可为蛔虫的种群遗传学、流行病学、宿主特异性、遗传结构、交配模式等研究提供有效的工具。
The purpose of this study was to develop microsatellite markers for Ascaris suum based on the whole genome.Genotyping was performed by PCR amplification and capillary electrophoresis.Twenty-five microsatellite loci that had polymorphisms and resulted in stable amplification were obtained.The number of alleles per locus ranged from 2 to 25.The observed heterozygosity(H O)and expected heterozygosity(H E)were 0 to 1 and 0.042 to 0.945,respectively.The polymorphism information content(PIC)was 0.040 to 0.921,with 20 pairs showing higher levels of polymorphisms.The frequency of null alleles(F)and inbred coefficient(F IS)ranged from 0 to 0.1971 and-0.1985 to 1,indicating loss of heterozygotes.Among the 25 pairs of microsatellite molecular markers,fifteen pairs of microsatellite markers conformed to Hardy-Weinberg equilibrium(HWE),and 10 loci showed deviations from HWE.These loci can also be stably amplified in A.lumbricoides.These newly developed microsatellite markers are effective tools to study population genetics,epidemiology,host specificity,genetic structure,and mating patterns of A.lumbricoides and A.suum.
作者
何晶晶
黄建华
牛红艳
周春花
吴小平
HE Jing-jing;HUANG Jian-hua;NIU Hong-yan;ZHOU Chun-hua;WU Xiao-ping(School of Life Science,Nanchang University,Nanchang 330031,China)
出处
《中国兽医杂志》
CAS
北大核心
2020年第9期11-14,19,共5页
Chinese Journal of Veterinary Medicine
基金
国家自然科学基金(81460318)。
关键词
猪蛔虫
全基因组
微卫星标记
多态性
Ascaris suum
genome-wide
microsatellite markers
polymorphisms
