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1株高效聚磷菌的分离鉴定及固定化应用 被引量:4

Isolation,Identification and Immobilization of a High-Efficiency Phosphorus Accumulating Bacteria
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摘要 为修复富营养化水体提供理论和实验依托,探讨了聚磷菌及固定化后对磷去除的影响,从氧化沟曝气池中筛选出1株高效聚磷菌W1,并经16S rDNA鉴定和系统发育分析,同时研究该菌株的除磷特性和固定化应用。结果表明,菌株W1确定为不动杆菌属(Acinetobacter sp.)。W1生长较为迟缓,20 h进入对数期,40 h后进入稳定期。当磷的质量浓度为1 mg/L时的除磷效果为好,除磷率为84.7%;适宜除磷pH为7~8;固定化W1颗粒在磷的质量浓度分别为1、5 mg/L时的处理效果分别为89.3%、93.1%;与游离W1相比处理效率分别提高4.6、15.6百分点;固定化W1与Langmuir吸附等温模型拟合效果较好(R^2=0.988)。 The theoretical and experimental support for the restoration of eutrophic water bodies was provided,the effect of phosphorus accumulating bacteria and immobilization on phosphorus removal was discussed.A high-efficiency phosphorus-accumulating bacteria W1 was screened from the oxidation ditch aeration tank,and the 16 S rDNA identification and phylogenetic analysis were conducted to study the phosphorus removal characteristics and immobilization application of the strain.The results showed that,the strain W1 was determined to be Acinetobacter sp..W1 grew slowly,entered the logarithmic phase at 20 h,and entered the stable phase after 40 h.When the mass concentration of phosphorus was 1 mg/L,the phosphorus removal effect was good,and the phosphorus removal rate was 84.7%.The suitable pH for phosphorus removal was 7~8.The treatment effects of immobilized W1 particles at phosphorus mass concentrations of 1 and 5 mg/L were 89.3%and 93.1%,respectively.The treatment efficiency was improved by 4.6%and 15.6%compared with the free strain W1.The immobilized W1 fitted the Langmuir adsorption isotherm model better(R!2=0.988).
作者 毛世丽 代群威 郭军 李相邑 李佳 黄云碧 MAO Shili;DAI Qunwei;GUO Jun;LI Xiangyi;LI Jia;HUANG Yunbi(Schoo of enironmend and resources,Soushurest Uniersity of science and lechnology;Fundamental Science on Nucleur Wastes and Enuironmenal Sufey Laboratory,Southuest Unitersiy of Science and Technology:Mianyang 621010,China)
出处 《水处理技术》 CAS CSCD 北大核心 2021年第1期59-63,共5页 Technology of Water Treatment
基金 国家自然科学基金面上项目(41877288) 国家自然科学基金重点项目(41831285)。
关键词 聚磷菌 筛选 固定化 应用 phosphorus accumulating bacteria screening immobilization application
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