摘要
目的针对梅毒低流行率人群,采用多表位融合片段策略以提高苍白密螺旋体(Tp)筛查试验的特异度,减少Tp假反应性结果。方法通过原核表达系统获得包含Tp47蛋白多个优势B细胞表位的多表位融合蛋白。随后将该多表位融合蛋白作为抗原用于建立双抗原夹心酶联免疫吸附试验(ELISA)法并检测96份梅毒阳性血清(1≤S/CO≤10)。将梅毒螺旋体颗粒凝集试验(TPPA)结果作为标准,将多表位融合蛋白的灵敏度和特异度与Tp47蛋白进行比较。结果多表位融合蛋白双抗原夹心ELISA法检测的灵敏度和特异度分别为90.32%和94.12%,Tp47蛋白双抗原夹心ELISA法检测的灵敏度为93.55%,特异度较低,为79.41%。多表位融合蛋白双抗原夹心ELISA法与TPPA结果的符合率为91.67%,高于Tp47蛋白的88.54%。结论多表位融合蛋白可有效提高梅毒血清学诊断的特异度。该研究为开发新一代具有较高特异度的梅毒诊断方法提供了思路。
Objective To adopt the multiple-epitope fusion fragment strategy to increase the specificity of anti-treponema pallidum(Tp)screening test for decreasing the Tp false reactivity results by aiming at the population of syphilis low prevalence rate.Methods The multi-epitope fusion protein containing multiple dominant B-cell epitopes of Tp47 protein were obtained by the prokaryotic expression system.Subsequently,the multi-epitope fusion protein was used as an antigen to establish an enzyme linked immunosorbent assay(ELISA)double antigen sandwich to detect 96 cases of syphilis positive serum(1≤S/CO≤10).Then,the TPPA results were used as the standard,and the sensitivity and specificity of the multi-epitope fusion protein were compared with Tp47 protein.Results The sensitivity and specificity of double antigen sandwich ELISA were 90.32%and 94.12%respectively.The sensitivity of Tp47 protein detected by ELISA double antigen sandwich was 93.55%,while the specificity was low,which was 79.41%.The coincidence rate with multiple-epitope fusion protein TPPA double antigen sandwich with the results of TPPA was 91.67%,which was higher than 88.54%in Tp47 protein.Conclusion The multi-epitope fusion protein can effectively increase the specificity of syphilis serum diagnosis,and provide an ideas for the development of a new generation of syphilis diagnostic methods with higher specificity.
作者
雷雪
林勇平
向波
杜丽枝
邓湘雪
刘斯琦
林润培
刘忠民
LEI Xue;LIN Yongping;XIANG Bo;DU Lizhi;DENG Xiangxue;LIU Siqi;LIN Runpei;LIU Zhongmin(Department of Clinical Laboratory,First Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510000,China;KingMed School of Laboratory Medicine,Guangzhou Medical University,Guangzhou,Guangdong 510000,China)
出处
《国际检验医学杂志》
CAS
2021年第2期197-201,共5页
International Journal of Laboratory Medicine
基金
广州医科大学大学生实验室开放项目(B185004171)。
