摘要
在生物、医学研究采用两血管阻断法(2-VO)建立脑缺血模型过程中,由于对动脉的非量化结扎,导致模型建立的重复性较差,组内个体差异较大且死亡率较高.本文以金属应变计为核心元件设计了血管阻断拉力仪,实现对结扎力的量化检测.从体外、体内两个层面以及体内的细胞分子水平、组织器官水平和整体水平进行生物学实验验证.结果表明:该拉力仪线性度、灵敏度受金属应变计基底参数、胶接工艺、电路参数、外观结构等因素影响;与非仪器结扎组比较,脑缺血模型动物死亡率降低了42.22%.实验动物脑血流量下降程度与结扎力递增呈对应关系;海马组织苏木素-伊红(HE)染色后光镜检测发现神经元排列松散,神经元数量减少乃至消失,神经元胞浆染色变淡及齿状回出现神经元固缩.不同结扎力组间比较显示,1.5 N组内大鼠海马组织形态个体差异最小;Morris水迷宫结果显示,与假手术组比,1.5 N结扎组、2.5 N结扎组大鼠逃避潜伏期显著增加(P<0.05),且1.5 N结扎组大鼠站台穿越次数显著下降(P<0.05),出现了明显的学习记忆障碍.证伪了实验室前期实验中2-VO大鼠结扎力越大,结扎效果越佳的理论.得出结扎力标准偏差越小结扎效果越好,且结扎力量化可控的结论.该血管阻断拉力仪应用于医药学基础实验研究具有重要意义.
In biological and medical research,two-vessel occlusion(2-VO)is often used to establish cerebral ischemia models,while due to the non-quantitative ligation of the artery,the model usually has poor reproducibility,large individual differences within the group,and high mortality.In this paper,a vascular blocking tension meter is proposed by taking metal strain gauge as the core component to realize the quantitative detection of ligation force.Biological experimental verification was carried out in vitro and in vivo,as well as in such aspects as cells and molecules level,tissues and organs level,and overall level.Results show that the linearity and sensitivity of the tension meter were affected by factors such as base parameters,bonding process,circuit parameters,and appearance structure of the metal strain gauge.Compared with the non-instrumental ligation group,the animal mortality of the cerebral ischemia model was reduced by 42.22%.The decrease in cerebral blood flow of the experimental animals corresponded to the increase in ligation force.After hematoxylin-eosin(HE)staining of the hippocampus,light microscopy results show that the neurons were loosely arranged,the number of neurons decreased and even disappeared,the cytoplasmic staining of the neurons became weak,and the dentate gyrus exhibited neuronal pyknosis.A comparison between different ligation force groups shows that the individual differences in the hippocampus morphology of the rats in the 1.5 N ligation force group were the smallest.Morris water maze results show that compared with the sham operation group,the escape latencies of the rats in the 1.5 N ligation group and the 2.5 N ligation group were significantly increased(P<0.05),and the number of platform crossing times in the 1.5 N ligation group was significantly decreased(P<0.05).There was an obvious learning and memory disorder.It was falsified that the greater the ligation force of the 2-VO rats was,the better the ligation effect was,which was obtained in our previous laboratory experiments.The conclusion was drawn that the smaller the standard deviation of the ligation force was,the better the ligation effect was,and the ligation force could be quantitatively controlled.The application of the vascular blocking tension meter to the basic experimental research of medicine is of significance.
作者
刘琳
王伟佳
霍炬
郇帅
赵瑛
LIU Lin;WANG Weijia;HUO Ju;HUAN Shuai;ZHAO Ying(College of Pharmacy,Harbin University of Commerce,Harbin 150076,China;School of Electrical Engineering and Automation,Harbin Institute of Technology,Harbin 150006,China;School of Basic Sciences,Harbin University of Commerce,Harbin 150028,China)
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2021年第2期184-190,174,共8页
Journal of Harbin Institute of Technology
关键词
血管阻断拉力仪
便携式
金属应变计
两血管阻断法
生物学实验
vascular blocking tension meter
portable
metal strain gauge
two-vessel occlusion
biological experiment
