摘要
目的:建立天花粉配方颗粒高效液相色谱-蒸发光散射法测定L-瓜氨酸的含量。方法:采用高效液相色谱法,以Inertsil ODS-SP柱(4.6 mm×250 mm,5μm)为色谱柱,水为流动相,采用蒸发光散射检测器检测。检测波长为270 nm;流速为0.5 mL•min^-1,载气流速为3.0 L•min^-1,飘移管温度为115℃。结果:L-瓜氨酸的峰面积的对数值与质量浓度的对数值线性关系良好,线性范围为0.05-1.00 mg•mL^-1,加标回收率为97.9%,相对标准偏差为1.0%。结论:该方法快速、简单、准确、重现性好,适用于天花粉配方颗粒中L-瓜氨酸的定量分析。
Objective:To establish a method for determination of L-citrulline in Tianhuafen Formula Granules by high performance liquid chromatography coupled with evaporative light scattering detection.Methods:The analytical column was Inertsil ODS-SP(4.6 mm×250 mm,5μm).The mobile phase was water,and the sample was determined by an evaporative light scattering detector with the flow rate 0.5 mL•min^-1.The flow rate of atmosphere was 3.0 L•min^-1 and the temperature of drift tube was kept at 110℃.Result:The logarithm correlation between peak area and the concentration of L-citrulline had good linearity in the range of 0.05-1.00 mg•mL^-1,and the average recovery was 97.9%.Conclusion:The method is a rapid,convenient,and effective method for qualitatively analysis of L-citrulline in Tianhuafen Formula Granules.
作者
周颖
祝清岚
昝珂
ZHOU Ying;ZHU Qing-lan;ZAN Ke(Zhejiang Institute for Food and Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine,Zhejiang Hangzhou 310053,China;National Institutes for Food and Drug Control,Beijing 100050,China)
出处
《中国药物评价》
2020年第6期448-450,共3页
Chinese Journal of Drug Evaluation
