摘要
目的:研究结扎诱发的大鼠牙周炎模型中炎症的动态变化。方法:选取健康雄性SD大鼠21只,随机分为7组,分别为正常对照组和结扎组A-F(结扎组A-F分别依次结扎1、3、5、7、14、21 d后处死)。除正常对照组各组均选用结扎丝于右侧上颌第一磨牙牙颈部结扎,建立大鼠实验性牙周炎模型,通过观测21 d内大鼠体重变化,探究结扎丝的介入对实验性牙周炎炎症过程的影响,通过软垢指数(DIS)、龈沟出血指数(SDI)及牙齿松动度(TM)的监测,评估结扎21 d内目标牙牙周炎症变化,为了更精确地评估炎症阶段牙槽骨的动态变化,使用CBCT观察牙槽骨吸收情况。结果:结扎组A-F在结扎后3 d体重均下降,第3天体重开始恢复上升。并且与正常对照组相比,结扎组3~21 d的体重增长率比较,差异无统计学意义(P>0.05),说明由于结扎丝的介入,大鼠会出现3 d的适应性现象,而在结扎3 d后结扎丝即对大鼠生长发育不会产生影响;结扎侧DIS在结扎后第1、3、5、7天较正常对照侧显著升高(P<0.05),且DIS值在前3 d呈上升状态,第5天开始逐渐下降,直至结扎第21天与正常对照组比较,差异无统计学意义(P>0.05);结扎侧SDI在第5天明显升高,第7天达到峰值后逐渐下降(P<0.05),直至结扎第21天与正常对照组比较,差异无统计学意义(P>0.05);结扎侧TM第7天开始出现变化,直至第21天逐渐上升,与正常对照组比较,差异有统计学意义(P<0.05);结扎组A、B、C的CEJ-ABC距离与正常对照组比较,差异无统计学意义(P>0.05),结扎组D、E、F的CEJ-ABC距离明显高于正常对照组(P<0.05),且F组与D、E组对比骨吸收更加显著(P<0.01)。结论:结扎诱导的大鼠实验性牙周炎在结扎后3 d为牙周炎始发期;结扎的3~7 d处于急性炎症期;14~21 d转为慢性炎症阶段,为慢性牙周炎的炎症进展期。本研究对结扎诱导的大鼠牙周炎发生发展过程进行了更加精细地定义,为后续对牙周炎模型的研究提供了新的方向,为更好更准确地进行临床研究奠定基础。
Objective:To study the dynamic changes of inflammation in rat models of periodontitis induced by ligation.Methods:Twenty-one healthy male SD rats were selected and randomly divided into 7 groups,namely normal control group and ligation group A-F(the ligation groups A-F were ligated 1,3,5,7,14 and 21 d respectively,and then sacrificed).In addition to the normal control group,ligature was used to ligate the neck of the right maxillary first molar.The experimental periodontitis model was established in rats.By observing the changes in rat body weight within 21 d,we explored the intervention of ligature wires on inflammation.The impact of the process,through monitoring the debris index simplified(DIS),Sulcus Bleeding Index(SBI),Tooth Mobility(TM),to evaluate the periodontal inflammation of the target tooth within 21 d of ligation.In addition,in order to more accurately assess the dynamic changes of the alveolar bone during the inflammatory phase,we use CBCT Observe the alveolar bone resorption.Results:The body weight of the ligation group decreased during the 3 d after ligation,and compared with the normal control group.There was no statistically significant difference in body weight growth rate in the ligation group from 3 to 21 d(P>0.05),indicating that due to the intervention of the ligation wire,the rat will have a 3-day adaptability phenomenon.The ligation of silk after day will not affect the growth and development of rats.The DIS on the ligated side was higher than that of the normal control on the 1st,3rd,5th,and 7th days after the ligation,respectively.The side increased significantly(P<0.05),and the DIS value increased in the first 3 days,and gradually decreased on the 5th day,until the 21st day after ligation,compared with the normal control group,the difference was not statistically significant(P>0.05);SDI on the ligation side increased significantly on the 5th day,and gradually decreased after reaching the peak on the 7th day(P<0.05),until there was no statistical difference between the ligation side and the normal control group on the 21st day(P>0.05);The day began to change and gradually increased until the 21st day,which was statistically significant compared with the normal control group(P<0.05);the CEJ-ABC distance of the ligation group A,B,and C was not statistically significant compared with the normal control group(P>0.05),the CEJ-ABC distance of the ligated group D,E,and F was significantly higher than that of the normal control group(P<0.05),and bone resorption resorption was more significant in group F compared with D and E groups(P<0.01).Conclusion:Three days after the ligation of the ligature,it affects the molar eating in rats,and induces inflammation.Ligation-induced experimental periodontitis is in the acute inflammatory phase during 3-7 days of ligation,and turns to chronic inflammatory phase in 14-21 d.Our This study provides presents a more precise definition of the occurrence and development of ligation-induced periodontitis in rats,which provides a new direction for the subsequent study of periodontitis models and provides a basis for better and more accurate clinical research.
作者
任百洁
刘悦
李雨阳
邹馨颖
孔晨
王雷
REN Bai-jie;LIU Yue;LI Yu-yang;ZOU Xin-ying;KON Chen;WANG Lei(Department of Periodontology, Hospital of Stomatology, Jilin University, Key Laboratory of Tooth Development and Jaw Remodeling and Regeneration of Jilin Province, Changchun, 130021, China)
出处
《海南医学院学报》
CAS
2021年第2期88-93,共6页
Journal of Hainan Medical University
基金
吉林省科技发展计划项目(20190303183SF)
吉林大学本科教学改革研究项目(2019XYB318)。
关键词
牙周炎
软垢指数
龈沟出血指数牙齿松动度
Periodontitis
Debris index simplified
Sulcus bleeding index
Tooth mobility
