摘要
实现低成本、规模化发酵培养青春双歧杆菌有赖于寻找新型、价格低廉的液体发酵培养基。以番茄、马铃薯、黄秋葵、紫薯、胡萝卜5种廉价易得的蔬菜作为筛选原料,分别提取其可溶性营养成分作为培养青春双歧杆菌的液体培养基,同时以当前国标和工业化生产所用的PYG、牛奶2种液体培养基为对照,在发酵培养0~72 h过程中,选取多个时间点进行动态跟踪,考察7种液体培养基对青春双歧杆菌的增殖效果及菌体生长规律。对照组的PYG、牛奶2种培养基在72 h内活菌浓度峰值分别为1.456×10^7 CFU·mL^-1、1.568×10^7 CFU·mL^-1,以黄秋葵、紫薯、马铃薯、胡萝卜为原料的蔬菜液体培养基,在72 h内活菌浓度峰值分别为6.832×10^7 CFU·mL^-1、2.671×10^7 CFU·mL^-1、1.600×10^7 CFU·mL^-1、4.320×10^7 CFU·mL^-1,对青春双歧杆菌的增殖效果均超过PYG、牛奶2种标准液体培养基。对7种培养基中的初始可溶性糖(碳源)、可溶性蛋白质(氮源)含量测定,结果显示2种营养物质在各种培养基中的含量及比例均存在明显差异。发酵培养青春双歧杆菌的结果显示,初始的可溶性糖和蛋白质的质量浓度与培养效果没有呈现典型的正相关,只有两者的利用率均高时才能获得较高的活菌浓度。以黄秋葵、紫薯、马铃薯、胡萝卜为原料的液体培养基均可以替代国标和工业化生产中常用的PYG、牛奶2种液体培养基,实现低成本发酵获取活性青春双歧杆菌。
The achievement of low-cost,large-scale fermentation of Bifidobacterium adolescentis relies on the development of new cost-effective liquid culture media. In this study,five vegetables,including tomato,potato,okra,purple potato,and carrot,were selected as the raw materials,the soluble nutrient components of which were subsequently extracted as the liquid culture media. Two kinds of liquid media,PYG and milk,which are commonly used in national standards and industrial production,were set as control. During the process of 0-72 h fermentation culture,multiple time points were selected for dynamic tracking,and the effects of the seven liquid media on the proliferation and population growth of Bifidobacterium adolescentis were evaluated. The peak values of viable bacteria in PYG and milk were 1. 456×10^7 CFU/mL and 1. 568×10^7 CFU/mL,respectively in 72 h. Those in the vegetable liquid media extracted from okra,purple potato,potato,and carrot were 6. 832×10^7 CFU/mL,2. 671×10^7 CFU/mL,1. 600×10^7 CFU/mL,and 4. 320×10^7 CFU/mL,all of which exceeded those in PYG and milk. Further results showed that in the initial content and ratio of soluble sugar( carbon source) and protein( nitrogen source) in the 7 culture media were remarkably different. The results of Bifidobacterium adolescentis fermentation showed that the initial mass concentration of soluble sugar and protein did not showa typical positive correlation with the culture effect of Bifidobacterium adolescentis,and a higher concentration of viable bacteria could be obtained only when the utilization ratio of both was high. The liquid media extracted from either okra,purple potato,potato,or carrot could replace PYG and milk to realize the cost-effective fermentation of viable Bifidobacterium adolescentis.
作者
张腾霄
王斌
蔡宏达
冯俊宇
王瑞祥
张世东
ZHANG Tengxiao;WANG Bin;CAI Hongda;FENG Junyu;WANG Ruixiang;ZHANG Shidong(Department of Food and Pharmaceutical Engineering,Suihua University,Suihua 152061,China)
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2020年第6期74-79,共6页
Journal of Henan University of Technology：Natural Science Edition
基金
黑龙江省省属高校基本科研业务费研究项目“工业发酵生产过程提高发酵产品安全性和节能节水技术研究”(KYYWF10236190201)
黑龙江省大学生创新创业训练计划项目“益生元物质的筛查及用于研制益生菌新型培养基的研究”(201810236002)。
关键词
双歧杆菌
蔬菜
液体培养基
液态发酵
Bifidobacterium adolescentis
vegetables
liquid media
liquid fermentation
