摘要
目的:研究舌鳞状细胞癌CAL27细胞中过表达多配体蛋白聚糖1(SDC1)对细胞迁移、侵袭、细胞周期和细胞中活性氧(ROS)水平的影响,阐明SDC1在舌鳞状细胞癌发生发展中的潜在作用机制。方法:前期实验中成功构建的稳定高表达ptt5-SDC1的人舌鳞状细胞癌CAL27细胞作为实验组,稳定转染ptt5空载体的CAL27细胞作为对照组,Western blotting法检测2组细胞中SDC1蛋白表达水平,细胞划痕实验检测2组细胞划痕愈合率,Transwell小室实验检测2组CAL27细胞中迁移和侵袭细胞数,流式细胞术检测2组不同细胞周期CAL27细胞百分率和细胞中ROS水平。结果:与对照组比较,实验组CAL27细胞中SDC1蛋白表达水平明显升高(P<0.05),细胞划痕愈合率明显降低(P<0.05),迁移和侵袭细胞数明显减少(P<0.01),G0/G1期细胞百分率明显升高(P<0.05),细胞中ROS水平明显升高(P<0.05)。结论:SDC1过表达能够抑制舌鳞状细胞癌CAL27细胞的侵袭和转移,其作用机制可能与细胞周期阻滞和介导细胞凋亡途径有关。
Objective:To study the effect of overexpression of syndecan-1(SDC1)on migration,invasion,cell cycle,and intracellular reactive oxygen(ROS)level of the tongue squamous cell carcinoma CAL27 cells,and to elucidate the potential mechanism of SDC1 in the occurrence and development of tongue squamous cell carcinoma.Methods:The tongue squamous cell carcinoma CAL27 cells stably highexpressing ptt5-SDC1 established successfully in the previous experiment were used as experimental group,and the CAL27 cells stably transfected with ptt5 empty were used as control group.Western blotting method was used to detect the expression levels of SDC1 protein in the cells in two groups.Cell scratch test was used to detect the healing rates of cell scratch in two groups;Transwell chamber assay was performed to detect the number of migration and invasion cells;flow cytometry was used to detect the percentage of CAL27 cells in different cell cycles and the levels of intracellular ROS.Results:Compared with control group,the expression level of SDC1 protein in CAL27 cells in experimental group was significantly increased(P<0.05),the healing rate of scratch of the cells was significantly reduced(P<0.05),the number of migration and invasion cells was significantly reduced(P<0.01),the percentage of cells in G0/G1 phase was significantly increased(P<0.05),and the inrracellular ROS level was significantly increased(P<0.05).Conclusion:SDC1 overexpression can inhibit the invasion and metastasis of tongue squamous cell carcinoma CAL27 cells,and its mechanism may be related to cell cycle arrest and the pathway mediating apoptosis.
作者
刘璐
张天夫
王晓峰
孔晨飞
LIU Lu;ZHANG Tianfu;WANG Xiaofeng;KONG Chenfei(Department of Stomatology,China-Japan Union Hospital,Jilin University,Changchun 130033,China;Scientific Research Center,China-Japan Union Hospital,Jilin university,Changchun 130033,China)
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2021年第1期59-65,共7页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金项目(81903881)
吉林省科技厅自然科学基金项目(20200201398JC)
吉林大学白求恩计划科研项目(2018B02)。
