摘要
目的建立同时快速测定补益类保健食品中人参皂苷和非法添加西地那非的方法。方法采用ACQUITY UPLC?BEN C18色谱柱(50 mm×2.1 mm,1.7 μm),流动相采用乙腈-0.1%乙酸水溶液以设定梯度洗脱,电喷雾电离(ESI)源,多反应监测(MRM)扫描方式,同时对补益类保健食品中的人参皂苷和非法添加的西地那非进行检测。结果 3种人参皂苷(人参皂苷Rg1、人参皂苷Rb1、人参皂苷Re)在0.2~20 μg·mL-1,西地那非在10~500 μg·mL-1均与测定值具有良好线性关系,相关系数均大于0.997。人参皂苷样品低、中、高浓度平均回收率在96.4%~109.4%,RSD在3.5%~6.9%;西地那非样品低、中、高浓度平均回收率在100.4%~103.0%,RSD在1.9%~2.8%。结论该方法具有操作简单、检验速度快、定性更可靠、灵敏度高、定量准确的优点,适用于测定补益类保健食品中的人参皂苷成分和非法添加西地那非的快速检测。
Objective To establish a rapid method to determine the ginsenoside and sildenafil in tonic health food.Methods ACQUITY UPLC? BEN C18 column (50 mm×2.1 mm,1.7 μm) was used.The mobile phase consisted of acetonitrile-0.1% acetic acid aqueous solution with gradient elution.Electrospray ionization (ESI) source and multiple reaction monitoring (MRM) scanning methods were used to simultaneous detecte ginsenosides and sildenafil.Results The 3 ginsenosides (ginsenoside Rg1,ginsenoside Rb1,and ginsenoside Re) had good linearity at 0.2-20 μg·mL-1.The sildenafil had a good linearity at 10-500 μg·mL-1.The correlation coefficients were bigger than 0.997.The average recovery of ginsenosides was at 96.4%-109.4%,and RSD was 3.5%-6.9%.The average recovery of sildenafil was 100.4%-103.0%,RSD was 1.9%-2.8%.Conclusion The method is simple,fast,reliable,highly sensitive and accurate,which is suitable for the determination of ginsenoside and illegally added sildenafil in tonic health food.
作者
岳磊
崔燕贞
刘娜娜
祝慧阳
李晓静
YUE Lei;CUI Yan-zhen;LIU Na-na;ZHU Hui-yang;LI Xiao-jing(Zhengzhou Institute for Food and Drug Control,Zhengzhou 450000;Henan University,Kaifeng Henan 475000;Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450000)
出处
《中南药学》
CAS
2021年第1期110-113,共4页
Central South Pharmacy
基金
2020年度河南省科技协会“i科普”科技志愿者服务项目。
