摘要
目的:从自由基来源和清除的角度探讨针刺对自发性高血压大鼠(SHR)延髓头端腹外侧区(RVLM)中氧化应激水平影响的潜在机制。方法:15只Wistar(WKY)大鼠为正常组,45只SHR大鼠随机分为高血压组、针刺组和非穴组。针刺组取“太冲穴”直刺,非穴组取非穴位浅刺,治疗1次/d,共14 d。采用遥测技术检测针刺对血压的影响;采用DHE染色检测针刺对RVLM中活性氧族(ROS)变化的影响;采用化学发光法检测针刺对RVLM中超氧化物歧化酶(SOD)、一氧化氮合酶(NOS),和NADPH氧化酶活性的影响;采用Western Blot检测针刺对RVLM中NADPH氧化酶、SOD、NOS各亚基或亚型蛋白表达的影响。结果:与正常组比较,高血压组大鼠收缩压显著增高(P<0.05),RVLM中ROS产生增多(P<0.05),NADPH氧化酶活性增高(P<0.05),其亚基gp91phox、p22phox和nNOS蛋白表达水平显著升高(P<0.05)。与高血压组和非穴组比较,针刺干预可显著下调SHR大鼠的收缩压(P<0.05),降低RVLM中ROS的水平(P<0.05),下调NADPH氧化酶活性(P<0.05)。各组中SOD和NOS活性,SOD1、SOD2和iNOS蛋白表达水平无显著改变。结论:针刺可显著改善SHR大鼠血压,降低RVLM中ROS的水平,其机制可能与下调NADPH氧化酶亚基gp91phox、p22phox、及NOS亚型nNOS的表达水平相关。
Objective:To explore the potential mechanism of acupuncture on oxidative stress level in the rostral ventrolateral medulla(RVLM)of spontaneously hypertensive rats(SHR)from the perspective of free radical source and elimination.Methods:A total of 15 Wistar(WKY)rats were in the normal group,and 45 SHR rats were randomly divided into a model group,an acupuncture group and a non-acupoint group.In the acupuncture group,“Taichong”acupoints were used for treatment once a day for 14 days.In the non-acupoint group received superficial needling was used.The effects of acupuncture on blood pressure were measured by telemetry;the effects of acupuncture on the changes of reactive oxygen species(ROS)in RVLM were detected by DHE staining;the effects of acupuncture on the activities of superoxide dismutase(SOD),nitrogen synthase(NOS)and NADPH oxidase activity in RVLM were detected by chemiluminescence;and the effects of acupuncture on the subunits or subunits expression of NADPH oxidase,SOD and NOS in RVLM were detected by Western Blot.Results:Compared with the normal group,the systolic blood pressure of the model group was significantly increased(P<0.05),ROS production in RVLM increased(P<0.05),the NADPH oxidase activity increased(P<0.05),and the protein expression levels of gp91phox,p22phox and nNOS were significantly increased(P<0.05).Compared with the model group and non-acupoint group,acupuncture intervention could significantly down-regulate the systolic blood pressure of SHR rats(P<0.05),the level of ROS in RVLM(P<0.05),and the activity of NADPH oxidase(P<0.05).There was no significant change in the SOD and NOS activities,the protein expression of SOD1,SOD2 and iNOS among each groups.Conclusion:Acupuncture could significantly reduce high blood pressure and ROS levels in the RVLM of SHR rats.The mechanism may be related to the down-regulation of the expression of NADPH oxidase subunits gp91phox,p22phox and NOS subtype nNOS.
作者
王雪蕊
郭玉红
徐霄龙
白云静
刘清泉
WANG Xuerui;GUO Yuhong;XU Xiaolong;BAI Yunjing;LIU Qingquan(Beijing Hospital of Traditional Chinese Medicine affiliated to Capital Medical University,Beijing 100029,China)
出处
《世界中医药》
CAS
2021年第1期77-81,共5页
World Chinese Medicine
基金
国家自然科学基金青年项目(82004292)
国家自然科学基金面上项目(81973608)
国家自然科学基金专项项目(62041701)
国家科技重大专项(2017XZ10305501)
科技部公共安全风险防控与应急技术装备专项(2020YFC0841600)。
关键词
高血压
针刺
氧化应激
延髓头端腹外侧区
大鼠
Hypertension
Acupuncture
Oxidative stress
Rostral ventrolateral medulla
Rats
