摘要
为了分析在健胃消食片中应用高效液相色谱法对橙皮苷含量进行测定的效果,并对产品质量进行考察,本文利用高效液相色谱法对橙皮苷含量进行测定,色谱条件设置为C18色谱柱,流动相为水-冰醋酸-甲醇,比例为61∶4∶35,测定波长为283 nm,流速为1.0 mL/min。结果显示,在测定过程中溶剂最佳选择为流动相,提取方法最佳为加热回流法,线性良好范围为2~18μL,线性相关系数r=0.9998,对橙皮苷含量进行计算,回收率为98.5%,相对标准偏差为0.35%。在健胃消食片中应用高效液相色谱法对橙皮苷含量进行测定较为有效,可对其质量进行有效控制。
In order to analyze the effect of applying high performance liquid chromatography to the determination of hesperidin content in jianwei xiaoshi tablets,and investigate the quality of the product.In this paper,high performance liquid chromatography is used to determine the content of hesperidin.The chromatographic conditions are set to C18 chromatographic column,the mobile phase is water-glacial acetic acid-methanol,the ratio is 61∶4∶35,the measurement wavelength at 283 nm,the flow rate is 1.0 mL/min.The results show that the best choice of solvent in the determination process is the mobile phase,and the best extraction method is heating reflux,with good linearity ranging from 2—18μL,linear correlation coefficient r=0.9998.The hesperidin content is calculated and the recovery rate is 98.5%,and the relative standard deviation is 0.35%.It is more effective to use high performance liquid chromatography to determine the content of hesperidin in jianwei xiaoshi tablets,and its quality can be effectively controlled.
作者
马文学
MA Wenxue(Gansu Linxia Pharmaceutical Inspection and Testing Center,Linxia,Gansu,731100,China)
出处
《质量安全与检验检测》
2020年第6期41-43,共3页
QUALITY SAFETY INSPECTION AND TESTING
