摘要
Background:Most species of Triatominae live exclusively in Latin America.However,one species,Triatom a rubrofasciata,has been recorded in the Americas as well as in various port areas in Africa and Asia.An increasing number of T.rubrofosciato have been reported in southern China in recent years.However,the origin of this invasive insect vector in China remains unknown,therefore,accurate identification and phylogenetic analysis of the bugs are urgently needed.Methods:A total of seven triatomine insect specimens were found and collected from Maoming City,Guangdong Province,China(GDMM)and Zhangzhou City,Fujian Province,China(FJZZ),respectively.The obtained insect vector specimens were observed under a dissecting microscope for morphological classification and then the genomic DNA was extracted,and the 16S ribosomal RNA(rRNA),28S rRNA as well as cytochrome oxidase subunit I(COI)genes of the species were amplified and sequenced.Subsequently,molecular phylogenetic analyses based on multiple alignments of the above genes were conducted in order to identify the species and determine the phylogenetic origin approximation accurately.Results:The triatomine insects collected from GDMM and FJZZ were identified as Triatom a rubrofasciata using morphological and genetic analyses.All of the Chinese T.rubrofosciato captured in FJZZ,GDMM and other localities in southern China,together with a Vietnamese and Brazilian strain,formed a new,cohesive clade.T.rubrofasciata in GDMM and FJZZ are likely derived from strains found in Vietnam or Brazil.Conclusions:To the best of our knowledge,this is the first record of the invasive insect T. rubrofasciata,which is likely derived from strains native to Vietnam or Brazil,in both Maoming City,Guangdong Province and Zhangzhou City,Fujian Province of China.A comparison of the DNA sequences of the 16 s rRNA,28 srRNA and COI genes confirmed the specific identification of T. rubrofasciata,and its potential origin in China is based on the phylogenetic analyses undertaken in this study.More targeted interventions and improved entomological surveillance are urgently needed to control the spread of this haematophagous insect in China.
