摘要
群体感应抑制剂(Quorum Sensing Inhibitors,QSIs)可以通过干扰群体感应(Quorum Sensing,QS)而有效降低病菌的感染性和毒力,并且不会胁迫致病菌使其产生耐药性,是具有前景的抗生素替代品。海洋微生物是新型QSIs的潜在来源。本研究利用紫色杆菌(Chromobacterium violaceum 026,CV026)模型评价QS抑制活性,在来源于南海中西部和印度洋深海沉积物样品的放线菌中筛选QS抑制活性菌株,并且根据形态学特征和16S rRNA基因序列鉴定活性菌株。通过筛选获得了菌株SCSIO 53717,其提取物在CV026模型中能够显著降低指示菌株的紫色菌素产量,并且在0~1000mg·mL^-1的有效浓度范围内不影响紫色杆菌的生长。菌株SCSIO 53717被鉴定为Kocuria属,是首次报道的具有QS抑制活性的Kocuria属菌株,因而具有进一步深入研究的价值。
Quorum Sensing Inhibitors(QSIs) are promising alternatives to antibiotics, which can reduce the infectivity and virulence of pathogenic bacteria by interruption of quorum sensing(QS) pathways. The strategy aimed at disarming rather than at eradicating bacterial pathogens, and imposed no strong selective pressure on bacteria that can lead to the development of resistance mechanisms. Marine microorganism is one of the most potential sources of novel QSIs. In the present study,actinomycetes strains, isolated from the deep-sea sediment samples obtained from the South China Sea and Indian Ocean, were tested by Chromobacterium violaceum 026(CV026) model, for screening for QS inhibitory strains. Strain SCSIO 53717 showed inhibitory activity against quorum sensing of CV026. The extract of SCSIO 53717 significantly reduced the production of CV026 violacein, and the extract of the strain showed no inhibitory effects on the growth of CV026 strain within the concentration range from 0 to 1000 mg·mL–1. This strain was assigned to the genus Kocuria based on morphological characteristics and 16 S rRNA gene sequence analysis. To the best of our knowledge, this is the first time that Kocuria is reported to be QS inhibitory active. This result indicates that strain SCSIO 53717 is worthy further studying.
作者
李艳群
陈柔雯
林宗豪
田新朋
尹浩
LI Yanqun;CHEN Rouwen;LIN Zonghao;TIAN Xinpeng;YIN Hao(Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;University of Chinese Academy of Sciences,Beijing 100049,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China;Guangdong Key Laboratory of Marine Material Mcdica,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China)
出处
《热带海洋学报》
CAS
CSCD
北大核心
2021年第1期75-81,共7页
Journal of Tropical Oceanography
基金
中国科学院战略性先导科技专项(A类)(XDA13020300)
南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0401)
广东省重点领域研发计划(2020B1111030004)。
