摘要
近年来,子宫内膜异位症(endometriosis,EMs)发病率明显增加,给女性身心健康带来极大危害。螺旋藻多糖(polysaccharides from Spirulina,PSP)是一类具有多重功效的天然生物大分子,具有抗炎、抗细胞增殖等多种功能,药理学上具有治疗EMs的潜力。为了探讨PSP对EMs大鼠血清指标的影响及分子机制,本实验首先通过水提法提取PSP,柱层析法测得纯度为89%;以SD大鼠为模式动物,采用自体移植术成功制备了大鼠EMs模型,通过饲喂不同剂量的PSP,观察对EMs的治疗效果;用Western blot和RT-PCR检测PI3K/Akt/mTOR通路蛋白及mRNA含量。结果表明:与对照组相比,模型组EMs可显著增加大鼠血清中TNF-α、VEGF、MMP-2的水平;与模型组相比,不同剂量的PSP均可缓解EMs症状,具体表现为EMs病灶减小,血清TNF-α、VEGF、MMP-2等含量的降低。模型组中,PI3K、AKT、mTOR在转录水平与蛋白水平表达量均显著升高,而PSP治疗组PI3K、AKT、mTOR在转录水平与蛋白水平表达量显著降低。研究结果提示PSP可能通过抑制PI3K/Akt/mTOR信号通路进而改善EMs症状。
In recent years,the incidence of endometriosis(EMs)has increased significantly,bringing great harm to women's physical and mental health.Polysaccharide from Spirulina(PSP)is a class of natural biological macromolecules with multiple functions.They have various functions such as anti-inflammatory and anti-cell proliferation,and also have pharmacological potential to treat EMs.To investigate the effects and molecular mechanism,the PSP with purity of 89%was obtained by water extraction and analytical methods.SD rats were used as model animals.In the rat EMs model,an EMs rat model was successfully prepared by auto-transplantation surgery.Therapeutic effects of different doses of PSP on EMs were observed.The protein and transcription level expression of PI3K/Akt/mTOR in EMs tissue was detected by Western blot and qRT-PCR methods respectively.The results showed that compared with the control group,the EMs in the model group significantly increased the expression levels of TNF-α,VEGF and MMP-2 in the rat serum.Compared to model group,different doses of PSP alleviated the symptoms of EMs,which are specifically manifested as the reduction of EMs lesions and the decreased expression levels of TNF-α,VEGF and MMP-2 in serum.In the model group,the protein and transcription expression levels of PI3K,AKT and mTOR increased significantly,while the expression of PI3K,AKT,mTOR in the PSP treatment group decreased obviously.The research results indicated that PSP could improve the symptoms of EMs by inhibiting the PI3K/Akt/mTOR pathway and reducing TNF-α,VEGF,and MMP-2 contents in serum.
作者
王猛
马浩天
关思宇
于杰
狄建兵
王愈
李润植
崔红利
WANG Meng;MA Hao-tian;GUAN Si-yu;YU Jie;DI Jian-bing;WANG Yu;LI Run-zhi;CUI Hong-li(Institute of Molecular Agriculture and Bioenergy,College of Agriculture,Shanxi Agricultural University;College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,China)
出处
《天然产物研究与开发》
CAS
CSCD
2021年第1期16-22,共7页
Natural Product Research and Development
基金
国家自然科学基金(31902394)
山西农谷建设科研专项(SXNGJSKYZX201906)
晋中市重点研发项目(Y192012)
山西省研究生教育创新项目(2019SY226)。
