摘要
该研究采用超声波辅助纤维素酶法提取泡桐花总黄酮。在单因素试验的基础上,选择酶解温度、超声功率、液料比和酶解时间进行Box-Benhnken试验设计,响应面法优化泡桐花总黄酮提取工艺,并测定其抗氧化能力。结果表明,泡桐花总黄酮最佳提取工艺条件为酶解温度50℃,超声功率240 W,液料比40∶1(mL∶g),酶解时间36 min。在该优化提取条件下,总黄酮的提取得率为7.82%,与模型预测值8.01%接近。抗氧化试验结果表明,泡桐花总黄酮对DPPH自由基、羟自由基和ABTS自由基的半抑制浓度(IC50)值分别为214.2μg/mL、200.7μg/mL和328.5μg/mL,在总黄酮质量浓度为800μg/mL时,总黄酮对三者的清除率分别为83.1%、75.4%和64.3%。
The total flavonoids were extracted from Paulownia fortunei flowers by ultrasonic-assisted cellulase method.Based on the single-factor test,enzymolysis temperature,ultrasonic power,liquid-solid ratio and enzymolysis time were selected for the Box-Benhnken test design.The extraction process was optimized by response surface methodology and the antioxidant capacity was determined.The results showed that the optimal extraction conditions were enzymolysis temperature 50℃,ultrasonic power 240 W,liquid to solid ratio 40∶1(ml∶g)and enzymolysis time 36 min.Under the optimal extraction conditions,the extraction yield of total flavonoids was 7.82%,which was close to the model prediction value of 8.01%.The results of the antioxidant test showed that the half inhibitory concentration(IC50)of P.fortunei flowers total flavonoids on DPPH radicals,OH radicals and ABTS radicals were 214.2μg/ml,200.7μg/ml and 328.5μg/ml,respectively.With the total flavonoid concentration of 800μg/ml,the clearance rates of total flavonoids to the three free radical were 83.1%,75.4%and 64.3%,respectively.
作者
吕亭亭
杨志华
陶娟
韩永红
蔡亮亮
叶翩翩
刘旭
LV Tingting;YANG Zhihua;TAO Juan;HAN Yonghong;CAI Liangliang;YE Pianpian;LIU Xu(Department of Pharmacy and Traditional Chinese Pharmacy,Jiangsu College of Nursing,Huaian 223005,China;The First Affiliated Hospital,Tianjin University of Traditional Chinese Medicine,Tianjin 300381,China;Affiliated Hospital of Nantong University,Nantong 226001,China)
出处
《中国酿造》
CAS
北大核心
2021年第1期197-202,共6页
China Brewing
基金
国家自然科学基金(81900528)
淮安市自然科学研究计划项目(HAB201717)
江苏省大学生创新训练项目(202014541002Y)。
关键词
泡桐花
总黄酮
超声辅助酶法
响应面法
抗氧化活性
Paulownia fortunei flowers
total flavonoids
ultrasonic-assisted cellulase method
response surface methodology
antioxidant activity
