摘要
【目的】研究危害中国新疆和田地区洛浦县设施番茄的病毒种类,为该区域番茄病毒病的科学防治提供依据。【方法】2019年秋季,在洛浦县设施蔬菜产区采集8份番茄疑似感病样品。利用已报道侵染番茄的7种主要病毒的特异性引物对采集的番茄疑似感病样品进行RT-PCR检测,对番茄褪绿病毒阳性样品的CP基因进行克隆和测序,进行同源性、地理来源和系统进化分析。【结果】从8份样品中检测到南方番茄病毒病(Tomato yellow leaf curl virus,STV)、番茄黄化曲叶病毒病(Tomato yellow leaf curl virus,TYLCV)、番茄褪绿病毒(Tomato chlorosis virus,ToCV),其检出率分别为100%、100%、87.5%,未检出烟草花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaic Cucumovirus,CMV)、番茄花叶病毒病(Tobacco mosaic virus,ToMV)、番茄斑驳花叶病毒(Tomato mottle mosaic virus,ToMMV)。对ToCV基因进行克隆与序列分析,中国新疆洛浦县分离物的CP基因的774个核苷酸序列与已报道的51个ToCV CP基因具有较高的同源性,与中国其它地区、日本、韩国、希腊、土耳其、巴西等地分离物一致性均在96%以上,中国新疆洛浦县采集病样分离的ToCV CP基因与中国北京、山东分离物的CP基因聚集在1个分支上,亲缘关系较近。【结论】新疆洛浦县设施番茄疑似病毒病植株的"黄化"现象,由STV、TYLCV和ToCV中的2种或2种以上病毒复合侵染所致,番茄褪绿病毒已传播至新疆和田地区。
【Objective】To find out the virus species that harm the facility tomato in Luopu,Xinjiang,and provide the basis for scientific prevention and treatment of tomato virus disease in this area.【Methods】In the autumn of 2019,8 tomato samples with suspected symptoms of virus disease in the protected vegetable producing area in Luopu were collected and the reported tomato viruses were detected by reverse transciption-PCR(RT-PCR)using specific primers of 7 main viruses.The CP gene of the positive sample ToCV was cloned and later sequenced.Meanwhile,homology,geographic origin and phylogeny analysis were conducted.【Results】The results showed that STV,TYLCV and ToCV were detected in 8 samples,and the detection rates were 100%,100%and 87.5%,respectively.TMV,CMV,ToMV and ToMMV were not detected.The 774 bp sequences of CP genes isolated in Luopu had high homology with 51 ToCV CP genes reported,and the nucleotide sequence of the CP gene of ToCV isolate from Xinjinag shared high identity(up to 95%)with those of the ToCV isolates from China,Japan,South Korea,Greece,Turkey and Brazil.【Conclusion】The results showed that the etiolation of tomato plants collected from Loupu county were jointly infected by two or more viruses including STV,TYLCV and ToCV.It is the first time report that ToCV spread to Hotan,Xinjiang and infected tomatoes there.
作者
孙晓军
周婷婷
玉山江·麦麦提
何伟
罗文芳
许建军
阿布都赛买提·吐尔送
SUN Xiaojun;ZHOU Tingting;Yushanjing Maimaiti;HE Wei;LUO Wenfang;XU Jianjun;Abudusaimaiti Tuersong(Research Institute of Plant Protection, Xinjinag Academy of Agricultural Sciences, Urumqi 830091,China;Agricultural Technology Extension Station of Hotan Prefecture, Hotan, Xinjiang 848000, China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2021年第1期99-106,共8页
Xinjiang Agricultural Sciences
基金
国家重点研发计划(2016YFD0201005)
自治区重点研发计划(2018B01002-1)。
关键词
病毒病
番茄褪绿病毒病
分子检测
系统进化
virus disease
tomato chlorosis virus(ToCV)
molecular detection
phylogenetic evolution
