摘要
通过检测唾液乳杆菌O2A发酵上清液及其所产乳酸菌素对沙门氏菌的抑菌活性,考察金属离子对乳酸菌素抑菌活性的协同作用及其机理。结果表明,单独的乳酸菌素和乳酸菌素-金属离子复配均对沙门氏菌有较好的抑菌效果;不同抑菌剂对沙门氏菌处理18 h后,细菌的生长曲线发生较大变化,且Zn2+(30 mmol/L)-乳酸菌素、Zn2+(30 mmol/L)-发酵上清液的抑菌效果较佳。扫描电子显微镜和透射电子显微镜结果表明,与标准沙门氏菌菌株相比,处理组的菌株细胞完整性均受到不同程度的破坏,破坏程度最严重的Zn2+(30 mmol/L)-乳酸菌素处理组,其抑菌机理可能是破坏细胞结构的完整性,使菌体细胞壁和细胞膜受到损伤,从而抑制细菌生长甚至杀死细菌。
In this paper,the antimicrobial activity of fermentation supernatant and bacteriocin produced by Lactobacillus salivarius O2A against Salmonella was studied.The effect of metal ions-bacteriocin against Salmonella and its mechanism were investigated.Alone bacteriocin and metal ions-bacteriocin had good bacteriostatic effects on Salmonella.The growth curve of different antimicrobial agents on Salmonella changed greatly after treatment for 18 h,and the bacteriostatic effects was better in the Zn2+(30 mmol/L)-fermentation supernatant and Zn2+(30 mmol/L)-bacteriocin.Compared with the standard Salmonella strain,the results of scanning electron microscope and transmission electron microscope show that the micromorphology of the treated strain was destroyed to varying degrees,among which the most serious damage was caused by the Zn2+(30 mmol/L)-bacteriocin.The mechanism of bacteriostasis may be to destroy the cell wall and cell membrane by destroying the integrity of the cell structure,thus inhibiting the growth of bacteria even killing bacteria.
作者
李光月
陈漪汶
李雪玲
Li Guangyue;Chen Yiwen;Li Xueling(College of Food Science,South China Agricultural University,Guangzhou 510642)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第1期65-71,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
广东省科技创新战略专项(重点领域研发计划项目)(2018B020206001)。
关键词
唾液乳杆菌
乳酸菌素
金属离子
沙门氏菌
协同抑菌
抑菌机理
Lactobacillus salivarius
bacteriocin
metal ions
Salmonella
synergistic bacteriostasis
mechanism of bacteriostasis
