摘要
采用二倍稀释法和牛津杯法测定太白韭乙醇提取物对金黄色葡萄球菌的抑菌活性,采用原子吸收光谱法、扫描电子显微镜技术等分析太白韭乙醇提取物对金黄色葡萄球菌胞外金属离子含量、生物大分子(蛋白质、核酸、多糖)含量、电导率、细胞壁完整性、菌体超微结构等的影响,采用高效液相色谱法分析太白韭乙醇提取物的化学组成,探讨其抑菌机理。太白韭乙醇提取物对金黄色葡萄球菌的最小抑菌质量浓度为15 mg/mL。用太白韭乙醇提取物处理后,金黄色葡萄球菌胞外K+、Ca2+、Na+、Mg2+离子含量显著增加,胞外蛋白质、核酸、多糖含量显著升高,菌体培养液电导率也显著增大。太白韭乙醇提取物能够严重破坏金黄色葡萄球菌的细胞形态,使部分菌体之间出现粘连、聚合现象。在太白韭乙醇提取物中共检出大蒜素和蒜氨酸2种含硫类化合物,尿苷、β-胸苷、腺苷和2'-脱氧腺苷4种含氮类化合物,以及芦丁、山柰酚、槲皮素和异鼠李素4种黄酮类化合物。太白韭乙醇提取物对金黄色葡萄球菌的抑菌活性可能与其中所含芦丁、槲皮素、大蒜素、山柰酚等化合物有关。
Antibacterial activity of ethanol extract from Allium prattii against Staphylococcus aureus was analyzed by broth dilution and Oxford cup methods.Effects of ethanol extract from A.prattii on contents of extracellular metal ions and biological macromolecules(i.e.proteins,nucleic acids and polysaccharides),conductivity of culture medium,integrity of cell walls and ultrastructure of S.aureus were investigated using atomic absorption spectrometry,scanning electron microscopy and so on.Phytochemicals of ethanol extract from A.prattii were quantified by high-performance liquid chromatography.And antibacterial mechanism of ethanol extract from A.prattii was discussed.The minimum inhibitory mass concentration of ethanol extract from A.prattii was 15 mg/mL.After S.aureus was treated with ethanol extract from A.prattii,contents of extracellular K+,Ca2+,Na+,Mg2+,proteins,nucleic acids and polysaccharides markedly increased,and conductivity of culture medium significantly improved.Ethanol extract from A.prattii seriously destroyed cell morphology.Two sulfur compounds(allicin and alliin),four nitrogen compounds(uridine,β-thymidine,adenosine and 2'-deoxyadenosine monohydrate)and four flavonoids(rutin,kaempferol,quercetin and isorhamnetin)were detected in ethanol extract from A.prattii.Antibacterial activity of ethanol extract from A.prattii against S.aureus was possibly attributed to its phytochemicals such as rutin,quercetin,allicin and kaempferol.
作者
潘振东
李璐
薛梦莹
张华峰
Pan Zhendong;Li Lu;Xue Mengying;Zhang Huafeng(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119;International Joint Research Center of Shaanxi Province for Food and Health Sciences,Xi'an 710119;National Engineering Laboratory for Resources Development of Endangered Crude Drugs in Northwest China,Xi'an 710119;Research Station of Se-enriched Foods in Hanyin County of Shaanxi Province,Xi'an 710119)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第1期318-326,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
陕西省重点研发计划项目(2018ZDXM-SF-006,2017GHJD-006)
中央高校基本科研业务费项目(GK201906006)。
关键词
太白韭
金黄色葡萄球菌
抑菌活性
化学成分
抑菌机理
Allium prattii
Staphylococcus aureus
antibacterial activity
chemical composition
antibacterial mechanism
