摘要
为全面了解和提升兽医系统实验室检测能力,2017—2019年青海省动物疫病预防控制中心连续3年组织州县级兽医实验室开展检测能力比对工作。根据每年疫病的流行态势,设置PCR、ELISA以及传统类检测等比对项目。结果显示:2017—2019年实验室总体准确率分别为50.00%、56.82%、74.47%,样品总正确率分别为90.75%、93.56%、93.48%,整体呈上升趋势;各实验室PCR、ELISA、传统类检测项目的正确率差异不显著(P>0.05);州级实验室的检测正确率明显好于县级(P<0.05)。结果表明,州县级实验室检测能力逐年提升,绝大多数实验室具备PCR、ELASA、传统类检测等检测能力,但部分县级实验室的个别项目检测能力有待提高。建议进一步加大经费投入,加强基层实验室管理、业务培训等,整体提升县级实验室的检测能力。
In order to identify and improve the detection capacity of veterinary system laboratories,the veterinary laboratories at county/prefecture level were organized and compared by Qinghai Animal Disease Prevention and Control Center for three consecutive years from 2017 to 2019.Such comparison projects as PCR,ELISA and other traditional detection techniques were set up according to the status of disease prevalence every year.The results showed that the overall accuracy rates of laboratories from 2017 to 2019 were 50.00%,56.82%and 74.47%,respectively,those of samples were 90.75%,93.56%and 93.48%,respectively,with an increasing trend in general;the accuracy rates of all the projects were slightly different(P>0.05)in all laboratories,and the accuracy rates of the laboratories at prefecture level were obviously higher than those at county level(P<0.05).In conclusion,the detection capacity of the laboratories at county/prefecture level was increasing year by year,most of the labs were able to carry out PCR,ELISA and some traditional detection items,while several laboratories needed to be improved in the above mentioned fields.In general,the detection capacity of the laboratories at county/prefecture level should be improved through increasing funds and inputs,strengthening management and operation training of the laboratories at grassroots level,etc.
作者
拉华
王谢忠
游潇倩
张茂华
阚威
孙生帧
马艳萍
刘蓉菁
魏梦君
张晓英
赵全邦
La Hua;Wang Xiezhong;YouXiaoqian;Zhang Maohua;Kan Wei;Sun Shengzhen;Ma Yanping;Liu Rongjing;Wei Mengjun;Zhang Xiaoying;Zhao Quanbang(Qinghai Animal Disease Prevention and Control Center,Xining,Qinghai 810001,China)
出处
《中国动物检疫》
CAS
2021年第2期58-62,共5页
China Animal Health Inspection
基金
青海省重大科技专项(2016.SF-A5)。
关键词
兽医系统实验室
检测能力
比对
青海省
veterinary system laboratory
detection capacity
comparison
Qinhai Province
