摘要
目的建立能测定补骨脂中5种成分含量的一测多评法(QAMS)。方法采用HPLC法,使用Zorbar RRHD SB-C 18(150 mm×2.1 mm,1.8μm)和Shim-pack XR-ODSⅢC 18(150 mm×2.0 mm,2.2μm)色谱柱,以乙腈-2 mL·L^-1冰醋酸为流动相,梯度洗脱;流速:0.4 mL·min^-1;柱温:45℃;检测波长:246 nm。以补骨脂素为参照物,建立其与异补骨脂素、新补骨脂异黄酮、补骨脂二氢黄酮和补骨脂酚的相对校正因子(RCFs),计算各成分含量,利用外标法与QAMS法测定5种成分的含量,并比较2种方法的含量差异。结果5种成分的质量浓度分别在3.20~320.23(r 1=0.9999),2.70~312.91(r 2=0.9999),2.20~219.45(r 3=1.0000),2.72~271.76(r 4=1.0000),1.60~320.68(r 5=0.9999)μg·mL^-1范围内线性关系良好,异补骨脂素、新补骨脂异黄酮、补骨脂二氢黄酮和补骨脂酚相对补骨脂素的RCFs重复性良好,QAMS法的计算结果与外标法测得结果无显著差异。结论QAMS法能准确测定补骨脂中5种成分的含量,可用于补骨脂中多指标的质量控制。
Objective To establish an evaluation method by multi-components quantitation with one marker(QAMS)for 5 components in Psoralea corylifolia.Methods Zorbar RRHD SB-C 18(150 mm×2.1 mm,1.8μm)and Shim-pack XR-ODSⅢC 18(150 mm×2.0 mm,2.2μm)were used as stationary phase with acetonitrile-2 mL·L^-1 glacial acetic acid as mobile phase in a gradient elution mode;flow rate 0.4 mL·min^-1;column temperature 45℃;detection wavelength 246 nm.Taking psoralen as reference,the relative correction factors(RCFs)of psoralen with isopsoralen,neobavaisoflavone,bavachin and bakuchiol were established,and the contents of each component were calculated.At the same time,the contents of the 5 components were determined by external standard method and QAMS,and the differences between the results were compared.Results The 5 components were in the linear ranges of 3.20-320.23(r 1=0.9999),2.70-312.91(r 2=0.9999),2.20-219.45(r 3=1.0000),2.72-271.76(r 4=1.0000)and 1.60-320.68(r 5=0.9999)μg·mL^-1,respectively,the RCFs of isopsoralen,neobavaisoflavone,bavachin and bakuchiol were repeatable.There were no significant difference between the results of QAMS and that of external standard method.Conclusion The contents of 5 components in Psoralea corylifolia can be accurately determined by QAMS,which can be used for the quality control of Psoralea corylifolia.
作者
段丽
祝清灿
汪娥
孙宜春
DUAN Li;ZHU Qingcan;WANG E;SUN Yichun(Sinopharm Tongjitang(Guizhou)Pharmaceutical Limited Company,Guizhou 550026,China)
出处
《西北药学杂志》
CAS
2021年第1期10-14,共5页
Northwest Pharmaceutical Journal
基金
国家中药标准化项目(编号:ZYBZH-C-GZ-10)
贵州省科技计划项目(编号:黔科合支撑[2019]2778号)。
关键词
补骨脂
一测多评
补骨脂素
相对校正因子
Psoralea corylifolia
multi-components quantitation with one marker(QAMS)
psoralen
relative correction factors(RCFs)
