摘要
本实验采用核磁共振波谱仪对煎炸油中极性组分含量的测定方法进行了研究。通过对核磁共振谱图中煎炸油的特征峰相对峰面积与极性组分含量之间的数学关系进行分析,从而建立了核磁共振波谱仪测定煎炸油的极性组分含量的方法。主要结论如下:核磁共振波谱仪测定煎炸油的特征峰相对峰面积与极性组分含量之间的线性回归方程为y=-8.3276 x+90.215,相关系数R^2=0.9773;核磁共振波谱仪重复测定的煎炸油样品中极性组分含量的相对标准偏差范围为1.72%~2.53%(小于5%),表明核磁共振波谱仪检测煎炸油极性组分含量的方法具有较好的重复性。同时本研究将此法与柱层析法进行对比,所得测定结果没有显著性差异,表明核磁共振波谱法可以替代柱层析法测定煎炸油中极性组分含量。
The content of polar components in frying oil was detected by Nuclear magnetic resonance spectrometer in this experiment.By analyzing the mathematical relationship between the characteristic peak relative peak area and polar component content of the fried oil detected by means of Nuclear magnetic resonance spectrometer,the method of Nuclear magnetic resonance spectrometer detecting the polar component content of fried oil has been developed.The following main conclusions were gained:the linear regression equation between the characteristic peak relative peak area and polar component content of the fried oil detected by Nuclear magnetic resonance spectrometer was y=-8.3276 x+90.215,and the correlation coefficient R^2=0.9773;The relative standard deviation range of the fried oil samples repeatedly determined by Nuclear magnetic resonance spectrometer was 1.72%to 2.53%,less than 5%,indicating that the polar component content of the fried oil detected by NMR technology was better repeatability.At the same time,the method with Column chromatography was compared with that in this study.It was found that there was no significant difference in the results obtained,showing that Nuclear magnetic resonance spectrometer can replace column chromatography in the determination of polar components in frying oil.
作者
王鸣洁
龙娟
杨昊
肖新生
易晶晶
Wang Mingjie;Long Juan;Yang Hao;Xiao Xinsheng;Yi Jingjing(Department of Chemistry and Biological Engineering,Hunan University of Science and Engineering,Yongzhou425199)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2021年第1期175-180,共6页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金青年项目(21801075)
湘南优势植物资源综合利用湖南省重点实验室开放基金(XNZW17C10,XNZW17C08)
大学生研究性学习和创新性实验计划项目(湘科院教发[2018]32号)。
关键词
煎炸油
NMR技术
柱层析法
极性组分
相关性
fried oil
nuclear magnetic resonance spectrometer
column chromatography
polar component
relevance
