摘要
为实现短程硝化稳定运行,采用MOE(Molecular Operation Environment)模拟分析了甲酸对氨氧化菌(AOB)及亚硝酸盐氧化菌(NOB)关键酶活性的影响,研究通过投加甲酸实现短程硝化反硝化稳定运行的可行性。MOE模拟结果表明,有甲酸存在的情况下,AOB与反硝化菌会逐渐增长并成为优势菌。MBBR中投加甲酸的实验结果表明,投加甲酸后亚硝氮积累率最高达到89.71%,平均亚硝氮积累率由23.71%增加到76.03%;氨氮去除率虽然在投加甲酸初期有所下降,但运行稳定后平均氨氮去除率可达到99.31%,表明甲酸的投加对氨氮的去除没有影响。对未投加甲酸和投加甲酸20 d的微生物样品进行高通量测序分析结果表明,投加甲酸20 d反应器中出现了亚硝化菌nitrosomonas和有反硝化作用的thermomonas,这一结果与MOE模拟分析结果一致。
In order to realize stable operation of shortcut nitrification,MOE(molecular operation environment)was used to simulate the effect of formic acid on the key enzyme activities of ammonia oxidizing bacteria(AOB) and nitrite oxidizing bacteria(NOB),and then the feasibility of stable operation of shortcut nitrification and denitrification by dosing formic acid was studied. The results of MOE simulation show that ammonia oxidizing bacteria and denitrifying bacteria will gradually grow and become the dominant bacteria in the presence of formic acid. The experimental results of dosing formic acid in MBBR showed that the highest nitrite accumulation rate after dosing formic acid reached 89.71%,and the average nitrite accumulation rate increased from 23.71% to 76.03%;although the ammonia removal rate declined at the initial stage when dosing formic acid,the average ammonia removal rate reached 99.31% after stable operation,indicating that the addition of formic acid has no effect on the removal of ammonia nitrogen. The results of microbial high-throughput sequencing analysis of microbial samples without formic acid and for 20 days after dosing formic acid showed that nitrosomonas and thermomonas appeared in the reactor after adding formic acid 20 days,which consistent with the results of MOE simulation analysis.
出处
《环境保护与循环经济》
2020年第12期14-16,共3页
environmental protection and circular economy
基金
沈阳市“高层次创新人才计划”项目(RC190366)
辽宁省科技厅项目(2019-ZD-0483)
辽宁省教育厅项目(LJC201907)
大学生创新创业训练计划项目(201910166192)
大学生基金项目[L(A)2019348]
关键词
甲酸
MOE
AOB
NOB
短程硝化
微生物群落结构
Formic acid
MOE
AOB
NOB
shortcut nitrification
microorganism community structure
