摘要
目的建立尼帕病毒假病毒动物感染模型,降低活病毒动物模型的生物安全风险。方法利用HIV骨架体系包装尼怕病毒假病毒,然后以不同剂量,不同的途径实验感染金黄地鼠,动态检测尼帕假病毒在金黄地鼠体内发光和分布情况。结果除灌胃(IG)外、腹腔注射(IP)、胸腔注射(IC)途径均能使金黄地鼠感染尼帕假病毒,以IP组的感染效果较好,假病毒感染后体内分布主要在心脏、脾脏、胰、肝脏,从第1d可以检出假病毒发光,第2d基本达到发光高峰,持续至6d后开始下降,11d发光消失。结论成功建立尼帕病毒假病毒金黄地鼠动物感染模型建立成功,可以部分代替尼帕病毒的活病毒试验,降低生物安全风险。
Objective To establish an animal model of infection with a Nipah pseudovirus in order to reduce the biosafety risks of infection with the Nipah virus.Methods An HIV backbone plasmid system was used to construct a Nipah pseudovirus,and golden hamsters were infected with different doses of the Nipah pseudovirus via different routes.The Nipah pseudovirus distribution in golden hamsters and luminescence were measured dynamically.Results:Golden hamsters were infected with the Nipah pseudovirus intraperitoneally(IP),subcutaneously(IC),and intragastrically(IG);the best route was IP.in vivo,the pseudovirus was mainly located in the heart,spleen,pancreas,and liver.Luminescence was detected from day 1 to day 10.Luminescence peaked on day 2 and remained at that intensity until day 6.Luminescence then decreased until it disappeared on day 11.Conclusion A model of Nipah pseudovirus infection was successfully created in golden hamsters.It can partially replace the Nipah virus test and reduce biosecurity risks.
作者
王晴
聂建辉
梁春南
张黎
赵晨燕
黄维金
王佑春
WANG Qing;NIE Jian-hui;LIANG Chun-nan;ZHANG Li;ZHAO Chen-yan;HUANG Wei-jin;WANG You-chun(National Institutes for Food and Drug Control,Key Laboratory of the National Health Commission for Research on the Quality and Standardization of Biotech Products,Key Laboratory of Biological Product Quality Research and Evaluation of National Medical Products Administration,Beijing,China 102629)
出处
《中国病原生物学杂志》
CSCD
北大核心
2020年第11期1272-1276,共5页
Journal of Pathogen Biology
基金
十三五传染病重大专项课题资助项目(No.2018ZX10731101-002-006,2018ZX10731101-002-009,2018ZX10731101-001-006,2017ZX10202102)
创新生物技术药评价及标准化关键技术研究项目(No.2018ZX09101001)。
关键词
尼帕病毒
假病毒
动物模型
金黄地鼠
Nipah virus
pseudotyped virus
animal model
golden hamster
