摘要
针对水体中低浓度微囊藻毒素(MC-RR和MC-LR)的富集、淋洗、洗脱等前处理环节,采用单因素响应面优化法探讨微囊藻毒素前处理的最佳条件。结果表明:采用体积分数为25%的甲醇水溶液能够取得最佳淋洗效果,由One Factor设计优化得到体积分数为75%的甲醇水溶液作洗脱剂,MC-RR和MC-LR最佳回收率为89.4%和90.7%。方法在0.100 mg/L~5.00 mg/L范围内线性良好,MC-RR和MC-LR的检出限分别为0.07μg/L和0.04μg/L,实际水样加标回收率分别为80.6%和84.9%,平行测定结果的RSD分别为1.6%和2.4%。
In order to get optimum conditions,single-factor response surface optimization method was used in the pre-treatment process of enriching,leaching and eluting low concentration of microcystins(MC-RR and MC-LR)in water.The results showed that it could get the best leaching effect with 25%methanol solution,the best recoveries of MC-RR and MC-LR were 89.4%and 90.7%by using 75%methanol solution as eluent which optimized by One Factor design.The method had good linearity in the range of 0.100 mg/L to 5.00 mg/L,the detection limits of MC-RR and MC-LR were 0.07μg/L and 0.04μg/L,the recoveries of actual water samples were between 80.6%and 84.9%,and the RSDs of duplicate detection were 1.6%and 2.4%,respectively.
作者
周世会
陈海龙
韩孟书
江帆
李秋华
ZHOU Shi-hui;CHEN Hai-long;HAN Meng-shu;JIANG Fan;LI Qiu-hua(Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province,Guizhou Normal University,Guiyang,Guizhou 550001,China;International Joint Research Centre for Aquatic Ecology,Guiyang,Guizhou 550001,China;Key Laboratory for Information and Computing Science of Guizhou Province,Guizhou Normal University,Guiyang,Guizhou 550001,China)
出处
《环境监测管理与技术》
CSCD
2021年第1期43-46,共4页
The Administration and Technique of Environmental Monitoring
基金
国家自然科学基金资助项目(U1612442)
贵州省科技厅基金资助项目(黔科合平台人才[2018]5805)
贵州省教育厅基金资助项目(黔教合KY字[2017]032)
贵阳市科技计划基金资助项目(筑科合同[2019]2-9)。
