摘要
文章通过设置盐度梯度,对平均体质量为(8.495±1.11)g的四川华吸鳅(Sinogastromyzon szechuanensis)开展了盐度耐受性研究,以期为该物种保护及人工养殖提供理论依据。结果表明,盐度对四川华吸鳅作用24、48、72和96 h的半致死浓度(LC50)分别为10.50、10.15、9.83和9.46 g·L^(-1),安全浓度为2.32 g·L^(-1)。四川华吸鳅经盐度胁迫4 h后,盐度小于7 g·L^(-1)各组的呼吸频率较初期下降,盐度大于7 g·L^(-1)各组的呼吸频率较初期显著升高(P<0.05),2 g·L^(-1)组的呼吸频率为(150.0±5.57)次·min-1,显著高于0 g·L^(-1)组(P<0.05)。在0~7 g·L^(-1)盐度胁迫下适应4 h,2 g·L^(-1)组的四川华吸鳅窒息点最低[(0.81±0.02)mg·L^(-1)];适应96 h后,各盐度组四川华吸鳅的窒息点均下降,0~1 g·L^(-1)组的窒息点最低[(0.60±0.06)mg·L^(-1)]。研究表明,四川华吸鳅具有一定的低盐度耐受力,窒息点相比一般淡水鱼类高。若利用呼吸频率来监测盐度对四川华吸鳅的安全浓度,建议不超过(150.0±5.57)次·min^(-1)。在运输中将盐度控制在0~1 g·L^(-1),有助于增强四川华吸鳅的耐低氧能力,提高成活率。
We measured the salinity tolerance of Sinogastromyzon szechuanensis with average body mass of(8.495±1.11)g,to provide theoretical basis for its conservation and artificial culture.The results show that the half lethal concentrations(LC50)of salinity at 24 th,48 th,72 nd and 96 th hour was 10.50,10.15,9.83 and 9.46 g·L^(-1),respectively.The safe concentration was 2.32 g·L^(-1).After 4 h of salinity stress in S.szechuanensis,compared with the initial stage,the respiration frequency in groups with salinities less than7 g·L^(-1) decreased,and the respiration frequency of groups with salinity higher than 7 g·L^(-1) increased significantly(P<0.05).The respiratory rate of 2 g·L^(-1) group was(150.0±5.57)times·min-1,significantly higher than that of the 0 g·L^(-1) group(P<0.05).Being adapted to(0–7)g·L^(-1) salinity stress for 4 h,the asphyxia point of S.szechuanensis in 2 g·L^(-1) group was the lowest[(0.81±0.02)mg·L^(-1)].After 96 h adaptation,the asphyxia point of S.szechuanensis in each salinity group decreased,and the asphyxia point of(0–1)g·L^(-1) was the lowest[(0.60±0.06)mg·L^(-1)].It is concluded that S.szechuanensis has low salinity tolerance,and its asphyxiation point is higher than that of common freshwater fish.The critical value of using salinity to monitor the salinity to the safe concentration of S.szechuanensis is(150.0±5.57)times·min^(-1).Therefore,the salinity should to be conctrolled as(0–1)g·L^(-1) in transportation,which is helpful to enhance the hypoxia tolerance and survival rate of S.szechuanensis.
作者
陈小江
左华丽
李伟
吴建顾
CHEN Xiaojiang;ZUO Huali;LI Wei;WU Jiangu(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Shanghai Haifeng Aquaculture Co.,Ltd.,Yancheng 224153,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2021年第1期76-81,共6页
South China Fisheries Science
基金
美国大自然保护协会(TNC)资助项目(NA/BEIJING/SWU110510)
江苏高校青蓝工程资助(苏教师〔2018〕12)
泰州市311高层次人才培养工程资助项目(2017Ш-804)
江苏农牧科技职业学院科研基金项目(NSF201711)
企业横向项目(11710120022)。
关键词
四川华吸鳅
盐度耐受性
半致死浓度
Sinogastromyzon szechuanensis
Salinity tolerance
Half lethal concentration
