摘要
【目的】梨二次成花(异常)在我国南方普遍发生,环境因子与植物遗传因子互作在该过程中的作用以及关键的遗传要素仍有待鉴定,为鉴定梨基因组中SPL(squamosa promoter-binding protein like)编码基因并研究其在二次成花诱导中的表达变化情况。【方法】前期课题组探索了通过摘叶诱导模拟二次成花的生理机制,测定了转录组变化过程,本研究基于已有的白梨(Pyrus bretschneideri Rehd.)基因组数据,从全基因组范围对SPL编码基因进行了鉴定,结合二次花诱导过程的转录组数据,挖掘参与该过程的候选基因。【结果】本研究共获得了34个典型的SPL蛋白家族成员,只有3个成员在目前的梨参考基因组上串联排列。系统进化分析将34个成员划归为8个进化类群,与其他物种SPL分类一致。砂梨‘丰水’二次成花诱导及未诱导材料的转录谱分析表明,34个SPL蛋白编码基因中仅两个显著差异表达,其中Pbr038289.1显著上调表达,而Pbr002303.1表达量显著下降。【结论】这2个蛋白有望成为深入研究梨成花过程调控的重要靶点。
【Objective】The secondary flowering(abnormal) of pears is common in southern China. The interaction between environmental factors and plant genetic factors in this process and the key genetic elements remain to be identified. The present paper aimed to identify SPL(SQUAMOSA promoter-Binding protein like) gens in the genome of Pyrus bretschneideri and studies its expression changes in secondary flower induction.【Method】In our previous study, we explored the physiological changes of simulating secondary flowering by leaf defoliation and had determined the transcriptomic changes of the process. Based on the existing genomic data of Pyrus bretschneideri Rehd., the SPL-encoding gene was identified from the whole genome scale, and the transcriptome data of the secondary flower induction process was combined to mine the candidate genes involved in the process.【Result】A total of 34 typical SPL protein family members were obtained, and only three members were arranged in tandem on the current pear reference genome. The phylogenetic analysis classified 34 members into 8 evolutionary groups, in consistence with other species’ SPL classifications. Transcriptional analysis of the secondary flowering induction and uninduced materials of ’Hosui’ showed that only two of the 34 SPL protein-coding genes were significantly differentially expressed, of which Pbr038289 was significantly up-regulated, while Pbr002303 was significantly decreased. 【Conclusion】These two proteins are expected to become important targets for in-depth study of the flowering regulation in pear.
作者
钟必凤
邓家林
李文贵
张全军
刘冬梅
曲么日布
ZHONG Bi-feng;DENG Jia-lin;LI Wen-gui;ZHANG Quan-jun;LIU Dong-mei;QUME Ri-bu(Horticultural Research Institute of Sichuan Academy of Agricultural Sciences,Key Laboratory of Horticultural Crops,Biology and Germplasm Enhancement in Southwest Regions,Ministry of Agriculture,Sichuan Chengdu 610066,China;Agriculture and Animal Husbandry Bureau of Jiangyou City,Sichuan Jiangyou 621700,China;Agriculture and Animal Husbandry Bureau of Puge Country,Sichuan Puge 615300,China)
出处
《西南农业学报》
CSCD
北大核心
2020年第12期2905-2911,共7页
Southwest China Journal of Agricultural Sciences
基金
四川省财政育种工程青年基金(2017QNJJ-008)
成都市科技项目(2015-NY02-00009-NC)。
关键词
SPL蛋白
梨
二次花
基因家族
SPL protein
Pear
Secondary flower
Gene family
