摘要
目的探讨半枝莲氯仿极性部位提取物(ECSB)调控linc01843逆转大肠癌5-FU耐药的作用机制。方法在5-FU干预HCT-8和HCT-8/5-FU细胞后,通过QPCR法检测linc01843的表达。用lipofectamine 3000将si-linc01843、negative control分别转染到HCT-8和HCT-8/5-FU细胞中去,用5-FU干预转染后的两株细胞,通过MTT法检测5-FU对转染si-linc01843、negative control后的两株细胞生长活力的影响。用ECSB、5-FU、以及ECSB联合5-FU干预HCT-8/5-FU细胞,通过MTT法检测其细胞活力;用5-FU、ECSB联合5-FU干预HCT-8/5-FU细胞,通过QPCR法检测linc01843的表达。结果在5-FU干预下,HCT-8和HCT-8/5-FU细胞中的linc01843表达均显著高于各自对照组(P<0.05)。在5-FU干预下,转染了si-linc01843的HCT-8和HCT-8/5-FU细胞均显著提高了其对5-FU的敏感性(P<0.05)。在HCT-8/5-FU细胞中,ECSB能显著逆转其对5-FU的耐药性(P<0.05),可显著降低HCT-8/5-FU细胞中linc01843的表达(P<0.05)。结论linc01843参与了5-FU耐药的过程,且ECSB逆转大肠癌5-FU耐药的作用机制之一是通过调控linc01843的表达。
Objective: To investigate the reversal effect of chloroform fraction of Scutellaria barbata D. Don(ECSB) on 5-FU resistance in colorectal cancer by regulating linc01843. Methods: Following treatment with 5-FU, the expression of linc01843 was detected both in HCT-8 and HCT-8/5-FU cells using QPCR analysis. Si-linc01843 and negative control were transfected into HCT-8 and HCT-8/5-FU cells using lipofectamine 3000, respectively. After transfection, HCT-8 and HCT-8/5-FU cells were treated with 5-FU and their cell viability was evaluated by MTT assays. Meanwhile,HCT-8/5-FU cells were treated with ECSB, 5-FU or ECSB combined with 5-FU, and the cell viability was also determined by MTT assay. Subsequently, HCT-8/5-FU cells were treated with 5-FU,ECSB combined with 5-FU, and the expression of linc01843 was evaluated by QPCR analysis. Results: 5-FU treatment significantly upregulated the expression of linc01843 in HCT-8 and HCT-8/5-FU cells(P<0.05). Both HCT-8 and HCT-8/5-FU cells transfected with si-linc01843 significantly increased their sensitivity to 5-FU(P<0.05). In addition, ECSB could efficiently reverse the resistance of HCT-8/5-FU cells to 5-FU(P<0.05), and significantly decrease the expression of linc01843 in HCT-8/5-FU cells(P<0.05). Conclusion: linc01843 was involved in the process of 5-FU resistance, and a potential mechanism of ECSB increasing the sensitivity of HCT-8/5-FU to 5-FU may be mediated via regulating the expression of linc01843.
作者
张铃
方翌
林久茂
蔡巧燕
魏丽慧
熊晓满
刘菲
郑小红
俞诗雅
林燕滨
ZHANG Ling;FANG Yi;LIN Jiumao;CAI Qiaoyan;WEI Lihui;XIONG Xiaoman;LIU Fei;ZHENG Xiaohong;YU Shiya;LIN Yanbin(Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Fujian Key Laboratory of Integrative Medicine on Geriatrics,Fuzhou,Fujian 350122,China;College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China)
出处
《福建中医药》
2021年第1期23-27,共5页
Fujian Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81704069,81703913)。
