摘要
为筛选阿维菌素高产菌株,提高阿维链霉菌的发酵单位,降低生产成本,本试验主要采用紫外诱变结合不同类型抗生素的双重筛选方法对出发菌株AW-8进行诱变选育。结果表明:共筛选出具有链霉素单抗高产菌株16株,正突变率为10.7%;筛选出具有利福平单抗高产菌株27株,正突变率为14.2%;筛选出具有链霉素和利福平双抗高产菌株6株,正突变率为5%;经遗传稳定性验证,获得1株具有链霉素和利福平双抗高产稳产菌株R4,比单一抗性菌株W7的效价高8.53%,比出发菌株AW-8的效价高20.92%。该方法是一种便捷有效的选育方式。
In order to screen out avermectin high-yield strains,improve the fermentation unit of Streptomyces avermectin and reduce the production cost,the dual screening method of UV mutation combined with different types of antibiotics was used to screen the original strain AW-8.The results showed that,a total of 16 strains with high production of streptomycin monoclonal antibody were screened out,the positive mutation rate was 10.7%;27 strains with high production of rifampicin monoclonal antibody were screened out,the positive mutation rate was 14.2%;6 strains with high production of streptomycin and rifampicin double antibody were screened out,the positive mutation rate was 5%.A high and stable streptomycin and rifampicin resistant strain R4 was obtained.The titer of R4 was 8.53%higher than that of W7 and 20.92%higher than that of AW-8.This method is a convenient and effective breeding method.
作者
吕苗苗
杨光耀
田平雅
张会萍
牛春
张萍
LYU Miao-miao;YANG Guang-yao;TIAN Ping-ya;ZHANG Hui-ping;NIU Chun;ZHANG Ping(Ningxia Tairui Pharmaceutical Limited Company,Yinchuan 750101,China)
出处
《黑龙江农业科学》
2021年第2期61-65,共5页
Heilongjiang Agricultural Sciences
关键词
阿维菌素
阿维链霉菌
紫外诱变
抗性筛选
avermectin
Streptomyces avermitilis
UV mutagenesis
resistance selection
