摘要
目的:采用网络药理学方法探究百合抗抑郁的作用机制。方法:通过检索中药系统药理学数据库与分析平台(TCMSP)、BATMAN等在线数据库并结合文献调研,构建百合成分数据库;通过SwissTargetPrediction平台预测各成分潜在作用靶点,构建“成分-靶点”网络;以抑郁症为背景从GeneCards、OMIM、DrugBank数据库获得疾病靶点;通过与百合各成分靶点比对获得百合及抑郁共有靶点;采用Cytoscape构建百合抗抑郁的“成分-靶点-通路”网络,并进行KEGG通路富集分析。结果:通过网络药理学方法筛选得到百合发挥抗抑郁作用的16个生物活性成分,这些成分可作用于88个抑郁症相关的靶标。其中百合皂苷可能是其抗抑郁主要活性成分。百合可通过多靶点(STAT3、AKT1、JUN、VEGFA、TNF、APP、MAPK14、ESR1、AR和HSP90AA1等)参与小胶质细胞激活或衰减及雌激素水平的调节发挥抗抑郁作用,其中晚期糖基化终末产物(AGE)/晚期糖基化终末产物受体(RAGE)信号通路、磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路、鞘脂信号通路、C型凝集素受体信号通路、雌激素信号通路可能是其抗抑郁关键途径。结论:预测了百合抗抑郁的活性成分和潜在靶点,为百合及其复方抗抑郁机制研究提供参考。
Objective:To explore the antidepressant mechanism of Lilii Bulbus by using network pharmacology.Methods:The database of Lilii Bulbus ingredients was constructed a by searching online databases such as the traditional Chinese medicine system pharmacology database and analysis platform(TCMSP),BATMAN and combining literature research.The SwissTargetPrediction platform was used to predict the potential targets of each component and build a″component-target″network.The GeneCards,OMIM,and DrugBank databases were used to get the disease targets.The common targets of Lilii Bulbus and depression are obtained by comparing with component target of Lilii Bulbus.Cytoscape was used to construct the″component-target-pathway″network of antidepressant in lily,and conduct KEGG pathway enrichment analysis.Results:Network pharmacology methods successfully identified 16 biologically active ingredients in Lilii Bulbus,which acted on 88 depression-related targets.Among them,Lilium saponin may be its main antidepressant component.Lilii Bulbus can participate in microglial activation or attenuation through multiple targets(including STAT3,AKT1,JUN,VEGFA,TNF,APP,MAPK14,ESR1,AR and HSP90AA1),and The regulation of estrogen levels exerts antidepressant effects,among which the AGE/RAGE signaling pathway,PI3K/Akt signaling pathway,sphingolipid signaling pathway,C-type lectin receptor signaling pathway,and estrogen signaling may be the key antidepressant pathways.Conclusion:This study successfully predicted the antidepressant active ingredients and potential targets of Lilii Bulbus,which provided a scientific basis for the study of antidepressant mechanism of Lilii Bulbus.
作者
何丹
黄建华
曾宏亮
黄小龙
舒骏
邹笃准
赵河
张水寒
HE Dan;HUANG Jian-hua;ZENG Hong-liang;HUANG Xiao-long;SHU Jun;ZOU Du-zhun;ZHAO He;ZHANG Shui-han(Hunan Academy of Chinese Medicine,Hunan University of Chinese Medicin,Changsha 410013,China)
出处
《中国现代中药》
CAS
2020年第12期1996-2005,共10页
Modern Chinese Medicine
基金
名贵中药资源可持续利用能力建设项目(2060302)
全国中药资源普查项目(财社〔2017〕66号)
国家中药标准化项目(ZYBZH-Y-HUN-21)
湖南省高新技术产业科技创新引领计划项目(2020SK2029)。
关键词
百合
抑郁
网络药理学
通路分析
百合皂苷
Lilii Bulbus
depression
network pharmacology
pathway analysis
Lilium saponin
