摘要
目的:建立火麻仁标准汤剂超高效液相色谱法(UPLC)指纹图谱。方法:水溶性成分指纹图谱Ⅰ采用以十八烷基硅烷键合硅胶为填充剂的CORTECS T3色谱柱(150 mm×2.1 mm,1.6μm);乙腈-0.1%甲酸溶液为流动相梯度洗脱。检测波长为254 nm;流速为0.20 mL·min^-1;柱温为30℃。脂溶性成分指纹图谱Ⅱ采用以十八烷基硅烷键合硅胶为填充剂(100 mm×2.1 mm,1.8μm);乙腈-水为流动相梯度洗脱。检测波长为205 nm;流速为0.25 mL·min^-1;柱温为30℃。结果:建立火麻仁标准汤剂水溶性和脂溶性成分的指纹图谱,水溶性成分标示出6个共有峰,脂溶性成分标示出4个共有峰,并分别对20批火麻仁标准汤剂UPLC指纹图谱进行相似度评价,与对照指纹图谱相似度均在0.90以上。结论:该方法简单、准确、重复性好,为制定火麻仁配方颗粒的质量控制标准提供参考。
Objective:The fingerprint analysis method of Cannabis Fructus standard decoction was established.Methods:For water soluble constituents a CORTECS T3 column(150 mm×2.1 mm,1.6μm)was used with the column temperature maintained at 30℃.The mobile phase was acetonitrile-0.1%formic acid solution for gradient elution.The detection wavelength was at 254 nm and the flow rate was 0.2 mL·min^-1.For lipophillic constituents a C 18 column(100 mm×2.1 mm,1.8μm)was used with the column temperature maintained at 30℃.The mobile phase was acetonitrile-water solution for gradient elution.The detection wavelength was at 205 nm and the flow rate was 0.25 mL·min^-1.Results:The Water soluble constituents and fat soluble constituents fingerprint spectrums of Cannabis Fructus standard decoction was obtained.There were 6 common peaks were identified in water soluble constituents.There were 4 common peaks were identified in fat soluble constituents.The similarity of fingerprint spectrum among 20 batches of standard decoction was also evaluated.The degree of identification with the control fingerprint was above 0.90.Conclusion:The fingerprint analysis method of Cannabis Fructus standard decoction is simple,accurate and reproducible,which provides a scientific reference for establishing the quality control specification of Cannabis Fructus formula granule.
作者
陈锦霞
曾杉
林碧珊
陈伟钢
吕豪辉
CHEN Jin-xia;ZENG Shan;LIN Bi-shan;CHEN Wei-gang;LYU Hao-hui(Sionpharm Group Guangdong Medi-word Pharmaceutical Co.Ltd.,Shunde 528303,China)
出处
《中国现代中药》
CAS
2020年第12期2063-2067,共5页
Modern Chinese Medicine
