摘要
GDP-甘露糖4,6-脱水酶(GDP-mannose 4,6-dehydratase, GMD)是生物体内参与多糖合成代谢的一个重要基因。本研究利用毛竹全基因组和转录组数据信息,对GMD基因家族进行细致分析,共鉴定出39个家族成员。毛竹GMD各家族基因的理化性质有一定差异,但所含甘露糖4,6脱水酶结构域进化相对保守。进化树分析将毛竹GMD基因家族分为3大类。该家族基因上游启动子序列存在多种光响应、环境胁迫、激素应答等有关顺式作用元件。同源模建显示毛竹GMD为同源二聚体蛋白质,两个亚基均有相同或催化功能。转录组数据分析发现,部分成员GMD基因在竹笋生长阶段基因高表达特征明显,表明其可能参与笋的生长发育。另外,GMD的表达受到不同激素的影响。本研究通过对GMD基因的生物信息学分析,有利于进一步明确毛竹GMD基因的功能及其在岩藻糖生物合成途径中的作用。
GDP-mannose-4,6-dehydratase(GMD)is an important gene involved in the synthesis and metabolism of polysaccharides in living organisms.In this study,we used the genome and transcriptome information of moso bamboo(Phyllostachys edulis)to analyze the GMD gene family by bioinformatics methods.The results indicated that the whole genome of moso bamboo contains 39 candidate genes.Phylogenetic tree analysis showed that GMD genes family could be divided into three major groups.Although the physical and chemical properties of PeGMD are different,the evolution of the domain containing mannose 4,6-dehydratase is relatively conservative.The upstream promoter sequence of the family gene has various cis-acting elements such as photoreaction,environmental stress,and hormone response.Homology modeling shows that GMD is a homodimeric protein,and both subunits have the same catalytic function.The transcriptome data analysis showed that the GMD family genes may be involved in the growth and development of bamboo shoots.The gene expression of GMD could be affected by different hormones.In this study,the bioinformatics analysis of GMD genes are helpful to further clarify the function of the GMD family and their roles in the fucose biosynthesis pathway.
作者
阮诗雨
张智俊
陈家璐
马瑞芳
朱丰晓
刘笑雨
Ruan Shiyu;Zhang Zhijun;Chen Jialu;Ma Ruifang;Zhu Fengxiao;Liu Xiaoyu(State Key Laboratory of Subtropical Forest Cultivation,Zhejiang A&F University,Hangzhou,311300;School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou,311300)
出处
《分子植物育种》
CAS
北大核心
2021年第2期459-468,共10页
Molecular Plant Breeding
基金
国家自然科学基金(No.31770721)资助。
