摘要
FoF1-ATPase is an active rotary motor,and generates three-ATP for each rotation.At saturated substrate concentration,the motor can achieve about 103 r.p.m,which means one motor can generate about 105 ATP molecules during 30 min.Here,we constituted a novel nanodevice with a molecular rotary motor and a“battery”,FoF1-ATPase and chromatophore,and presented a novel method of sandwich type rotary biosensor based on εsubunit with one target-to-one motor,in which one target corresponds 105 ATP molecules as detection signals during 30 min.The target such as NT-proBNP detection demonstrated that this novel nanodevice has potential to be developed into an ultrasensitive biosensor to detect low expressed targets.
基金
This work is supported by the National Basic Research Program of China(973 Program)under grant No.2013CB932804
the National Natural Science Foundation of China under Grant No.11574329 and 11322543
the Science and Technology Planning Project of General Administration of Quality Supervision,Inspection and Quarantine(AQSIQ)of PR China under Grant No.2015IK011
AQSIQ industrial public service scientific research project of the Ministry of Science and Technology of P.R.China under Grant No.201410049
The YS101 type high sensitive detector of chemiluminescence was manufactured by Yishang Innovation Technology Co.,Ltd.
