摘要
为研究茉莉酸甲酯(methyl jasmonate,MeJA)对双孢蘑菇个体大小的影响,将不同浓度的MeJA溶液采前喷施双孢蘑菇。结果表明,经50μmol/L MeJA处理后,双孢蘑菇的平均单菇质量和伞直径、厚度均显著增加(P<0.05),并提前1 d长至采收期大小。50μmol/L MeJA处理后的第1、3天细胞色素P450氧化酶活力显著增加(P<0.05),第2天内切-β-1,4-葡聚糖酶活力显著增加(P<0.05)。对双孢蘑菇菌伞进行转录组测序,处理第1天和第0天相比,50μmol/L MeJA处理的双孢蘑菇中差异基因数为525个(P<0.05,|log2Fold Change|>1),显著高于空白组和乙醇组。GO富集分析显示MeJA可能通过影响富集在细胞过程、细胞、催化活性等与生长发育相关的GO条目中基因的差异表达促进双孢蘑菇个体增大。差异基因筛选的结果表明,MeJA可能通过上调基因AGABI2DRAFT_43781、下调基因AGABI2DRAFT_136361、AGABI2DRAFT_188444和AGABI2DRAFT_194024促进双孢蘑菇个体增大。本实验为MeJA促进双孢蘑菇个体增大提供了数据支撑和理论依据。
In order to study the effect of methyl jasmonate(MeJA)on the size of Agaricus bisporus,different concentrations of MeJA were sprayed on the mushroom before harvest.The results showed that compared to the control group,50μmol/L MeJA significantly increased the average mass of individual mushroom bodies and the diameter and thickness of pilei,and advanced the time to reach the harvest size by one day.The activity of cytochrome P450 oxidase significantly increased on day 1 and day 3(P<0.05),and the activity of endo-β-1,4-glucanase significantly increased on day 2(P<0.05)compared to the control group.According to the transcriptome sequencing,525 genes in the pilei of MeJA treated A.bisporus were found to be differentially expressed between day 0 and day 1,significantly more than those found in the control and ethanol treated groups(P<0.05,|log2Fold Change|>1).Gene ontology(GO)enrichment analysis showed that MeJA may increase the size of A.bisporus by affecting the differential expression of genes enriched in GO items related to growth and development such as cellular process,cell,and catalytic activity.The gene screening results showed that MeJA could up-regulate the gene AGABI2DRAFT_43781,and down-regulate the genes AGABI2DRAFT_136361,AGABI2DRAFT_188444 and AGABI2DRAFT_194024 to increase the size of A.bisporus.This study provides experimental and theoretical basis that MeJA increases the size of A.bisporus.
作者
宋媛
胡秋辉
苏安祥
裴斐
马高兴
马宁
杨文建
SONG Yuan;HU Qiuhui;SU Anxiang;PEI Fei;MA Gaoxing;MA Ning;YANG Wenjian(Key Laboratory of Grains and Oils Quality Control and Processing,Collaborative Innovation Center for Modern Grain Circulation and Safety,College of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing 210023,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第4期130-137,共8页
Food Science
基金
国家自然科学基金面上项目(31672213,32072293)
江苏高校优势学科建设工程资助项目(PAPD)。
关键词
双孢蘑菇
茉莉酸甲酯
个体大小
转录组测序
差异表达基因
Agaricus bisporus
methyl jasmonate
individual body size
transcriptome sequencing
differentially expressed genes
