摘要
目的TMCO1是一种新型的钙离子通道,与多种疾病有关。因其功能和性质尚不清楚,限制了对它的应用。研究TMCO1的性质,以利于疾病的治疗及药物的研究。方法应用TMCO1质粒转染HeLa细胞,培养30 h,分离内质网(ER)细胞膜,再用电生理Patch单通道的方法在不同细胞内外液中记录TMCO1的膜通道电流,最后采用Origin8.0统计及分析数据结果TMCO1可选择性通过K+和Na+,K+和Na+的选择性无差异;未加2-ABP的对照组电流是(2±0.5)pA,加入2-ABP的实验组电流为(1.3±0.1)pA;未加雷尼丁的对照组电流是(2±0.5)pA,加入雷尼丁的实验组电流为(2.2±0.2)pA;结论TMCO1通道对K+和Na+的选择性相似,且2-ABP和雷尼丁对TMCO1通道无影响。
Objective TMCO1 is a new type of calcium channel,which is related to many diseases.Its application is limited because its function and nature are unclear.This study aimed to examine the properties of TMCO1 in an attempt to facilitate the treatment of diseases and drug research.Methods HeLa cells were transfected with TMCO1 plasmid,and cultured for 30 h.The endoplasmic reticulum(ER)cell membrane was then separated,and the membrane channel currents of TMCO1 recorded in different intracellular and extracellular fluids using the electrophysiological Patch single channel method.0 rigin8.0 was used,finally,for statistical analysis.Results TMC01 could selectively pass K+and Na+,and there was no significant difference in the selectivity of K+and Na+.The current was(2±0.5)pA in the control group without 2-ABP,a calcium channel inhibitor,and that was(1.3±0.1)pA in the experimental group with 2-ABP.The current was(2±0.5)pA in the control group without ranitidine,a calcium channel agonist,and that was(2.2±0.2)pA in the experimental group with ranitidine.Conclusion The selectivity of TMCOl channel to K+and Na+is similar,and 2-ABP and ranedin have no effect on TMCO1 channel.
作者
杨宇秀
YANG Yuxiu(Pharmacy Department,Jiangxi Medical College,Shangrao,Jiangxi,334000,China;Basic Medical College,Nanchang University,Nanchang,330031,China)
出处
《医学分子生物学杂志》
CAS
2021年第1期58-61,共4页
Journal of Medical Molecular Biology
基金
江西省教育厅科学技术研究项目(No.171210)。
