摘要
探讨了复合酶解法制备的鲍鱼水解肽(AHP)的理化性质以及抗氧化、抗炎、调节免疫的作用效果。以自由基清除能力为依据,研究了其抗氧化能力;以对LPS诱导小鼠肺泡巨噬细胞(MH-S)白细胞介素与肿瘤坏死因子α(TNF-α)分泌活性的影响为依据,研究其抗炎作用;经口灌胃给予小鼠0.02、0.10、0.20 g/kg·bw三个剂量组的AP,测定小鼠体重、脏器/体重比值、迟发型变态反应、碳廓清和NK细胞活性变化,验证其对免疫功能的影响。结果表明,本研究的鲍鱼水解肽具有较强的清除DPPH自由基、ABTS自由基和超氧阴离子自由基能力,1.0 mg/mL的鲍鱼水解肽对DPPH自由基、超氧阴离子自由基的清除率分别可达到84.06%、69.74%,0.10 mg/mL的鲍鱼水解肽对ABTS自由基的清除率可达到62.41%;10μg/mL AP组显著抑制LPS诱导MH-S细胞分泌IL-1β、IL-6、TNF-α活性(P<0.05),50、100μg/mL AP组抑制效果极显著(P<0.01);中剂量AP组显著增强NK细胞活性(P<0.05);高剂量AP组显著促进小鼠的单核-巨噬细胞碳廓清功能(P<0.05),极显著增强NK细胞活性(P<0.01),从而达到调节免疫的作用。结论:鲍鱼水解肽具有较好的抗氧化性,同时具有抗炎和免疫调节作用。
Abalone hydrolytic peptide(AHP)was prepared from abalone meat by complex enzymatic method,and its anti-inflammatory and immunity enhancing effects were discussed.Method:The antioxidant of AHP was studied and free radical scavenging ability was taken as index,the anti-inflammatory effect of mouse alveolar macrophages(MH-S)was studied by interleukin and tumor necrosis factor-α(TNF-α),and the body weight,organ/body weight ratio,delayed allergy,carbon clearance and NK cell activity of mice were measured to study the effect of AAP(0.02,0.10 and 0.20 g/kg,respectively)on immune function.Results:AHP had strong ability to scavenge DPPH free radical,ABTS free radical and superoxide anion free radical,the scavenging rates of DPPH free radical and superoxide anion free radicals by 1.0 mg/mL AHP were 84.06%and 69.74%,respectively,and the scavenging rate of ABTS free radical by 0.10 mg/mL AHP was 62.41%.10μg/mL AP group significantly inhibited the secretion of IL-1β,IL-6 and TNF-αin MH-S cells induced by LPS(P<0.05),50,100μg/mL AP group were highly significant(P<0.01).Middle dose AHP group significantly increased NK cell activity(P<0.05),high dose AP group significantly promoted monocyte macrophage carbon clearance function(P<0.05),significantly enhanced NK cell activity(P<0.01),so as to achieve immune regulation.Conclusion:The AHP has good antioxidant activity,anti-inflammatory and immunomodulatory effects.
作者
王敏
卢赛
张曾亮
邹圣灿
丁伟
李丽杰
王尚龙
WANG Min;LU Sai;ZHANG Zengliang;ZOU Shengcan;DING Wei;LI Lijie;WANG Shanglong(Qingdao Yisheng Kanghe Biopharmaceutical Co.,Ltd.,Qingdao 266100,China;College of Traditinal Chinese Medicine,Inner Mongolia Medical University,Hohhot 010110,China)
出处
《食品工业科技》
CAS
北大核心
2021年第5期282-288,共7页
Science and Technology of Food Industry
基金
科技部国家重点研发计划资助项目(2018YFC0311203)。
关键词
鲍鱼水解肽
自由基清除率
抗炎
免疫调节
abalone hydrolytic peptide
free radical scavenging rate
anti-inflammatory
immunomodulatory
