摘要
大豆亲脂蛋白(SLP)是解决大豆分离蛋白水合特性和界面特性等功能特性问题的关键。采用多光谱、热分析和凝胶电泳等技术研究了大豆亲脂蛋白热诱导解离缔合行为,并对其自组装纳米颗粒进行表征。结果显示,80~90℃是SLP热处理过渡带,在热处理温度低于80℃时,SLP能基本保持天然构象而无显著性变化,在热处理温度高于90℃时,SLP二级构象发生显著性改变。在90℃处理20 min时,SLP蛋白分子结构解聚,且伸展至最大程度,表面疏水性增加,随后自组装形成粒径约为110 nm的稳定单分布纳米颗粒体系。电泳分析结果显示,解离的亚基通过二硫键和疏水相互作用重新聚集成中间聚集体,导致分子间聚集程度增大、构象稳定性增强。本研究可为SLP专用大豆蛋白粉及其在食品领域的开发应用提供理论支撑。
Soybean lipophilic protein(SLP)is a highly hydrophobic protein rich in phospholipids,which is the key to solve the functional defects of soybean protein isolates,such as hydration properties and interfaces properties,etc.The dissociation-association behavior and self-assembly nanoparticles of SLP induced by heating were characterized by some modern technology means,which were the ultraviolet spectrum,fluorescence spectrum,circular dichroism spectrum,Fourier transform infrared,thermal analysis and gel electrophoresis techniques.The results showed that the heating treatment transition zone of SLP was 80~90℃,where the SLP can basically maintain a natural structure without significant changes when the heating treatment temperature was below 80℃.While the secondary conformation of SLP was changed significantly when the heating treatment temperature was above 90℃.The molecular structure of the SLP was dissolved and extended to maximum extent,with increased surface hydrophobicity at 90℃foR^(2)0 min.Subsequently,the SLP self-assembled to form a stable single-distributed nanoparticle system with a particle size of about 110 nm.The dissociate subunits were reassembled into intermediate aggregates by interaction of disulfides and hydrophobic interaction,resulting in an increase of the degree of intermolecular aggregation and structural stability.The research result would provide theoretical support for the development of SLP-specific powder and its application in the food field.
作者
孙冰玉
刘琳琳
石彦国
朱秀清
张娜
曾剑华
SUN Bingyu;LIU Linlin;SHI Yanguo;ZHU Xiuqing;ZHANG Na;ZENG Jianhua(College of Food Engineering,Harbin University of Commerce,Harbin 150076,China)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2021年第2期346-354,共9页
Transactions of the Chinese Society for Agricultural Machinery
基金
黑龙江省自然科学基金联合引导项目(LH2020C060)
黑龙江省“百千万”工程科技重大专项(2019ZX08B01-04)
黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCT-2020217)
关键词
大豆亲脂蛋白
解离缔合行为
自组装纳米颗粒
热诱导
soybean lipophilic protein
dissociation-association behavior
self-assembly nanoparticles
heating induction
