摘要
采用三因素三水平响应面实验优化碱性蛋白酶的水解参数。通过Tricine-SDS-PAGE电泳分析对乳清蛋白水解物(whey protein hydrolysate,WPH)进行分析。经超滤得到分子量范围分别为≥10 ku和<10 ku的乳清蛋白水解物。将收集到的水解物分别添加到MRS培养基中,对Lactobacillus paracasei(L.paracasei)LX5进行培养。分别检测活菌数及多糖产量。结果表明:两个分子量范围的水解物均可促进L.paracasei LX5活菌数的增长和胞外多糖(exopolysaccharides,EPS)的产量。与添加分子量高于10 ku的水解物相比,添加分子量低于10 ku的水解物,菌株活菌数为280.33 CFU/mL,EPS产量为241.24 mg/L,其促生长作用及胞外多糖的产量明显高于前者。
Whey protein was hydrolyzed by alkaline protease.Optimizing the technological conditions of alkaline protease by response surface method contained three factors and three levels,which was based on the single factor experiment.Whey protein hydrolysate was analysed by Tricine-SDS-PAGE electrophoresis analysis and collected by ultrafiltration.The hydrolysates with molecular weight higher than 10 ku and lower than 10 ku were obtained.Two parts of hydrolysates collected were added to MRS medium respectively.The results showed that the number of living bacteria and the production of polysaccharides from L.paracasei LX5 were promoted by both of the hydrolysates.And the hydrolysates below 10 ku were more effective than those above 10 ku.The number of viable bacteria was 280.33 CFU/mL and the yield of exopolysaccharide was 241.241 mg/L in the medium supplemented with the hydrolysates below 10 ku.
作者
白英
殷佳棋
兰秀芬
王纯玮
BAI Ying;YIN Jiaqi;LAN Xiufen;WANG Chunwei(Food Science and Engineering College,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处
《中国乳品工业》
CAS
北大核心
2021年第2期14-18,23,共6页
China Dairy Industry
基金
内蒙古自治区自然科学基金(2019MS03046)
国家自然基金(31760460)。
关键词
乳清蛋白
水解
副干酪乳杆菌
胞外多糖
whey protein
hydrolyze
Lactobacillus paracasei
exopolysaccharides
