摘要
使用氯金酸和柠檬酸三钠回流法合成出荧光吸收峰在520 nm的金纳米颗粒,并与罗丹明B修饰合成探针。金纳米颗粒与罗丹明B发生荧光共振能量转移(FRET)作用使罗丹明B的荧光淬灭。在加入毒死蜱之后,毒死蜱及其水解产物能有效取代罗丹明B,使荧光恢复。金纳米颗粒探针对毒死蜱及其水解产物的浓度响应区间为(0~0.1)mm,低至0.1 nm也有响应,且荧光变化迅速,从而能够快速有效地检测超痕量有机磷农药毒死蜱及其水解产物。
A new assembled chlorpyrifos and its hydrolusate sensor based on rhodamine B(RhB)-modified Au nanoparticles(AuNPs)was developed by the reactions of chloroauric acid(HAuCl4)and sodium citrate under reflux conditions and modified by RhB.Due to the Fluorescence Kesonance Energy Transfer(FRET)effect,the emission at 520 nm of AuNPs can be quenched.With the addition of chlorpyrifos(CP)or its hydrolysate diethylphosphorothioate(DEP),the RhBs on the AuNPs were replaced along with the fluorescence recovery and enhancement.Such a fluorescence quenching and enhancement process of RhB-AuNPs system may have promising applications in chemical and biological sensors.
作者
王彦
余海
王利杰
袁欢欢
石道飞
WANG Yan;YU Hai;WANG Lijie;YUAN Huanhuan;SHI Daofei(School of Chemistry and Chemical Engineering,Anhui Key Laboratory of Functional Coordination Compounds,Anqing Normal University,Anqing 246133,China)
出处
《安庆师范大学学报（自然科学版）》
2021年第1期6-10,共5页
Journal of Anqing Normal University(Natural Science Edition)
基金
安徽省自然科学基金(1408085QB32)
安徽省高校优秀青年人才基金重点项目(2013SQRL059ZD)。
关键词
金纳米颗粒
荧光共振能量转移
荧光探针
gold nanoparticle
fluorescence kesonance energy transfer effect
fluorescence sensor
