摘要
为了鉴定从桉树根际筛选到的微生物的抑草潜力和抑草成分,以桉树根际土壤中筛选到的一株真菌为材料,利用生物测试验证菌株发酵液的抑草潜力,利用GC-MS对菌株发酵液抑草成分进行验证。结果表明,不同浓度菌株发酵液对稗草的根长、株高和干重都有显著的抑制作用,对稗草根长抑制率最大为稀释50倍时,抑制率为7.55%;对稗草株高和干重抑制率最高为原液,抑制率分别为56.53%和25.46%。GC-MS鉴定共检测到86个物质,含氮化合物最多,占59.10%,其次为羧酸及酯类化合物,占19.26%。抑草物质生物测试结果表明,邻香豆酸、柠檬醛和胡椒碱在低浓度时对莴苣的根长和株高都有很强的抑制作用。表明该菌株能够产生多种化感物质的结构类似物,使得菌株发酵液具有很强的抑草作用,具有开发为微生物除草剂的潜力。
To identify the herbicidal potential and constituents of microorganisms from Eucalyptus rhizosphere,a strain of fungi from the rhizosphere soil of Eucalyptus was used as material.The herbicidal activity of the strain fermentation broth was identified by bioassay,and the components of fermentation broth were further verified by GC-MS.The results showed that the fermentation broth of the strain at different concentrations had significant inhibition on the root length,plant height and dry weight of barnyard grass.The inhibition rate of the fermentation broth on the root length of barnyard grass was the highest(7.55%)under 50 times of diluted solution.The highest inhibition rate of barnyard grass height and dry weight was 56.53%and 25.46%by undiluted fermentation broth,respectively.GC-MS results showed that a total of 86 substances were detected,of which the largest proportion of compounds was nitrogen compounds accounting for 59.10%,followed by carboxylic acids and esters accounting for 19.26%.The bioassay results showed that o-coumaric acid,citral and piperine could inhibit the root length and plant height of lettuce at low concentrations.These results indicated that the strain could produce a variety of allelochemical analogues compounds,which made the strain fermentation broth have a strong weed-inhibition,and a great potential to be developed as a microbial herbicide.
作者
张奇
张清旭
庞晓敏
王海斌
叶江华
贾小丽
何海斌
Zhang Qi;Zhang Qingxu;Pang Xiaomin;Wang Haibin;Ye Jianghua;Jia Xiaoli;He Haibin(School of Tea and Food Science,Wuyi University,Wuyishan Fujian 354300;Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,Fujian Agriculture and Forestry University,Fuzhou 350002;College of Life Sciences,Longyan University,Longyan Fujian 364012)
出处
《中国农学通报》
2020年第35期106-112,共7页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金“稗草根系分泌物中水稻化感作用诱导成分分析及其诱导机理研究”(31370380),“化感水稻抑草圈形成的土壤微生物变化与调控机制研究”(31070447)
福建省教育厅中青年教师教育科研项目“外源诱导提高水稻化感的作用机理研究”(JAT190786)。
关键词
桉树
抑草真菌
发酵液
化感物质
GC-MS
Eucalyptus
weed-inhibiting fungi
fermentation broth
allelochemical
GC-MS
