摘要
为了研究玉米与拟南芥光敏色素C(Phytochrome C,PHYC)功能的异同,以及2个玉米PHYC基因在光形态建成和避荫性反应中的作用,克隆了玉米2个PHYC基因,用生物信息学方法分析玉米PHYC蛋白结构域,与常见物种PHYC蛋白进行系统进化分析,并利用转基因技术在拟南芥中验证玉米PHYC基因的功能。结果表明,从玉米B73自交系中克隆了ZmPHYC 1和ZmPHYC2基因。它们的编码区长度为3405 bp,编码具有1135个氨基酸残基、分子质量为126.14 ku和126.07 ku的蛋白质。ZmPHYC1和ZmPHYC2蛋白结构域与拟南芥和水稻的PHYC蛋白相似,包含1个PAS_2结构域、1个GAF结构域、1个PHY结构域、2个PAS重复结构域、1个组氨酸激酶A结构域和1个类组氨酸激酶ATP酶结构域。氨基酸序列同源性分析发现,二者与高粱PHYC同源性最高。在拟南芥中过量表达ZmPHYC 1和ZmPHYC2可以互补红光下拟南芥phyC-2突变体的长下胚轴表型,并且在持续红光和蓝光条件下均能促进幼苗的去黄化反应,在避荫条件下抑制下胚轴的伸长生长。在促进幼苗的去黄化反应和抑制避荫反应方面,ZmPHYC1的作用优于ZmP HYC2。
In order to study the similarities and differences of PHYC functions between maize and Arabidopsis and the functions in photomorphogenesis and shade avoidance of two maize PHYC genes,we cloned two maize PHYC genes,analyzed the functional domains of maize PHYC and phylogenetic tree of PHYC proteins maize and common plant species by bioinformatics,and verified the functions of two maize PHYC genes in Arabidopsis by transgenic techniques.The results showed that the coding regions of ZmPHYC 1 and ZmPHYC 2 from maize inbred line B73 were cloned.The coding region length was 3405 bp,and encoded a protein with 1135 amino acid residues with molecular weight of 126.14 ku and 126.07 ku.Similar to the PHYC proteins of Arabidopsis and rice,ZmPHYC1 and ZmPHYC2 contained a PAS_2 domain,a GAF domain,a PHY domain,two PAS repeat domains,a histidine kinase A domain and a histidine kinase like-ATPase domain.Amino acid sequence homology analysis showed that ZmPHYC1 and ZmPHYC2 had the highest homology with the sorghum PHYC protein.ZmPHYC 1 and ZmPHYC 2 could not only complement long hypocotyl phenotype caused by Arabidopsis phyC-2 mutant under red light,but also promote the de-etiolation of seedlings under continuous red and blue light conditions,and inhibit the elongation of hypocotyl under shade condition.For promotion of seedling de-etiolation and inhibition of shade avoidance,ZmPHYC 1 play more important role than ZmPHYC 2.
作者
丁武思
陈士瞻
刘磊
樊晓聪
丁梦月
孙广华
王立建
杨建平
DING Wusi;CHEN Shizhan;LIU Lei;FAN Xiaocong;DING Mengyue;SUN Guanghua;WANG Lijian;YANG Jianping(College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China;Henan Police College,Zhengzhou 450046,China)
出处
《河南农业科学》
北大核心
2021年第1期16-26,共11页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(31871709)
河南省技术创新引导专项(182106000050)
北京市自然科学基金重点项目(6151002)。
关键词
玉米
拟南芥
光敏色素C
去黄化反应
避荫性
Zea mays
Arabidopsis thaliana
Phytochrome C
De-etiolation
Shade avoidance
