摘要
研究天麻健株及病株内真菌群落结构差异,为查明天麻病害的发生机制提供线索。利用传统培养法和高通量测序技术分析天麻健株及病株块茎内真菌群落结构的变化。培养法分析结果表明,健株及病株天麻块茎真菌共涵盖3门14属,其中健株天麻中存在木霉属(Trichoderma)、踝节菌属(Talaromyces)等8个属;病株天麻中存在枝孢菌属(Cladosporium)、链格孢菌属(Alternaria)、镰刀菌属(Fusarium)等10个属。通过高通量测序技术共获得1692个OTUs(Operational taxonomic units),通过对物种进行分类可知,在门水平上,健株及病株天麻块茎的真菌主要类群基本一致,主要有子囊菌门(Ascomycota)、担子菌门(Basidiomycota)等,但健株和病株天麻各类群真菌相对丰度存在明显差异;在属水平上,健株与病株天麻块茎真菌群落组成及相对丰度差别很大,其中,土赤壳属(Ilyonectria)、柔膜菌属(Helotiales)和镰刀菌属(Fusarium)可能是引起天麻病害的致病菌群。可见,传统培养法和高通量测序技术2种方法均显示健株与病株天麻内真菌群落结构存在显著差异,天麻病害发生与其内真菌群落结构变化相关,提示可通过早期添加优势益生菌防控天麻病害。
The study of the differences in fungal community structure in healthy and diseased Gastrodia elata provides clues for the investigation of the mechanism of Gastrodia disease.Traditional culture method and high-throughput sequencing technology were used to analyze the changes of fungal community structure in healthy and diseased plants tubers.The results of culture analysis showed that the healthy and diseased Gastrodia tubers fungi covered 3 phyla and 14 genera.Among them,there were 8 genera including Trichoderma and Talaromyces in healthy Gastrodia.There were 10 genera including Cladosporium,Alternaria and Fusarium in the Gastrodia elata strain.A total of 1692 OTUs(operational taxonomic units)were obtained.The classification of species showed that at the phylum level,the main groups of fungi in the healthy and diseased groups of Gastrodia were basically the same,mainly including Ascomycota and Basidiomycota,but there were obvious differences in the relative abundance of fungi in healthy and diseased tubers of Gastrodia elata.At the genus level,the composition and relative abundance of fungi healthy and diseased Gastrodia were very different.Among them,Ilyonectria,Helotiales and Fusarium may be the pathogenic bacteria group that caused diseases of Gastrodia.It can be seen that both the traditional culture method and the high-throughput sequencing technology show that there are significant differences in the fungal community structure between healthy and diseased Gastrodia elata.The occurrence of Gastrodia elata disease is related to the change of the fungal community structure in Gastrodia elata,suggesting that it can be prevented by adding superior probiotics in the early stage.
作者
柏秋月
邓百万
解修超
张彩妮
刘兰兰
袁敏谦
BAI Qiuyue;DENG Baiwan;XIE Xiuchao;ZHANG Caini;LIU Lanlan;YUAN Minqian(School of Biological Science and Engineering,Shaanxi University of Technology/Shaanxi Provincial Engineering Research Center of Edible&Medicinal Fungi,Hanzhong 723001,China)
出处
《河南农业科学》
北大核心
2021年第1期92-100,共9页
Journal of Henan Agricultural Sciences
基金
陕西省科技创新科技工程项目(2019HBGC-19)。
关键词
天麻
病害
多样性
真菌群落
高通量测序
Gastrodia elata
Disease
Diversity
Fungal community
High-throughput sequencing
