摘要
【目的】筛选获得对辣椒疮痂病菌具有拮抗效果的内生细菌,并对其生防机制进行初步探究,为辣椒疮痂病的生物防治提供菌株资源和理论依据。【方法】以课题组构建的内生细菌库中的268株菌株为研究对象,采用抑菌圈法筛选获得辣椒疮痂病菌的拮抗菌株;通过生理生化特性测定、16S rDNA序列和gyrB基因序列分析明确拮抗菌株的分类地位;通过PCR扩增检测拮抗菌株的脂肽类物质合成基因。【结果】从268株内生细菌中筛选获得189株对辣椒疮痂病菌具有不同拮抗效果的菌株,占筛选总菌株数的70.52%,其中菌株B10(3)对辣椒疮痂病菌的抑菌效果最好,培养4 d时抑菌圈半径为21.0 mm。综合菌株B10(3)的生理生化特性测定、16S rDNA序列和gyrB基因序列系统发育鉴定结果,推断该菌株为解淀粉芽孢杆菌(Bacillus amyloliqufaciens)。菌株B10(3)的代谢产物对辣椒疮痂病菌具有拮抗作用,且能扩增出8个脂肽类物质合成基因(ituC、yndG、srfAB、srfAA、yndJ、bmyB、bioA和fenD)的目的条带。【结论】解淀粉芽孢杆菌菌株B10(3)对辣椒疮痂病菌具有较好的拮抗效果,且具备合成脂肽类物质的能力,具有良好的应用前景。
【Objective】Screening out the antagonistic strains against Xanthomonas euvesicatoria,and their biological control mechanism were preliminarily explored,so as to provide strain resources for biological control against X.euvesicatoria.【Method】There were 268 bacteria strains in endophytic bacteria library constructed by the research group.They were used to screen out the biocontrol bacterial strains against X.euvesicatoriaby inhibition zone method.And classification status of a biocontrol strain was determined by determination of physiological and biochemical characteristics,16S rDNA identification and gyrB gene sequence analysis,and PCR amplification was used to detect whether it contained lipopeptide synthesis gene.【Result】A total of 189 strains were found to inhibit X.euvesicatoria with various levels of effects from 268 endophytic bacteria strains,accounted for 70.52%of the total selected strains.The strain B10(3)had the best antagonistic effects,and the radius of bacteriostatic zone was 21.0 mm at 4 d of culture.Based on the physiological and biochemical characteristics,16S rDNA sequence and gyrB genes sequence analysis,the strain was identified as Bacillus amyloliqufaciens.The metabolites of this strain had antagonistic effect on X.euvesicatoria,and it could amplify 8 target bands of lipopeptide synthesis genes(ituC,yndG,srfAB,srfAA,yndJ,bmyB,bioA and fenD).【Conclusion】The strain B10(3)of B.amyloliqufaciens has good antagonistic effect on X.euvesicatoriawith the ability to synthesize lipopeptide,it has a good potential application prospect.
作者
詹梦琳
陈长江
罗晓华
胡方平
蔡学清
ZHAN Meng-lin;CHEN Chang-jiang;LUO Xiao-hua;HU Fang-ping;CAI Xue-qing(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Computer and Information Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Agricultural and Rural Bureau of Lianjiang County,Fuzhou City,Lianjiang,Fujian 350500,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2020年第12期2945-2951,共7页
Journal of Southern Agriculture
基金
国家重点研发计划项目(2017YFD0201106-02)
福建农林大学科技创新项目(KFA17541A)。
关键词
辣椒疮痂病菌
生防细菌
解淀粉芽孢杆菌
脂肽类合成基因
Xanthomonas euvesicatoria
biocontrol bacteria
Bacillus amyloliqufaciens
lipopeptide synthesis gene
