摘要
目的通过咪喹莫特(IMQ)乳膏涂抹诱导产生银屑病样皮损的小鼠模型,观察人源脐带间充质干细胞(hUCMSCs)对其整体和局部作用效果。方法C57BL/6小鼠随机分为对照组、模型组和hUC-MSCs组,模型组和hUC-MSCs组每日定时在小鼠裸露背部涂抹5%IMQ乳膏62.5 mg,对照组涂抹凡士林。在开始涂抹前1天(0 d)和涂抹后的第6天,对照组和模型组sc 200μL的生理盐水,hUC-MSCs组每只小鼠sc 2×10^(6)个hUC-MSCs。采用银屑病样皮损面积和疾病严重指数(PASI)评分标准观察银屑病样小鼠模型皮损变化情况;通过HE染色观察表皮层厚度,角质层角化程度及炎性细胞浸润程度;采集小鼠的静脉血,试剂盒法检测血清白细胞介素-6(IL-6)的浓度,评估小鼠炎症反应。结果hUC-MSCs组与同期模型组比较,皮损症状轻,通过PASI积分趋势图可见,hUC-MSCs能延缓皮损出现,同时减缓皮损严重程度,加快皮损部分的恢复;HE染色显示,hUC-MSCs组角化不全的细胞明显减少,红细胞外溢真皮层增生和炎性细胞浸润程度明显低于模型组,造模后6 d表皮层厚度显著低于模型组(P<0.01);hUC-MSCs组血清IL-6浓度在造模后的第12天显著低于模型组。结论银屑病样小鼠模型sc hUC-MSCs后能延缓银屑病的发生,并减轻银屑病症状,加快银屑病样皮损部分的恢复,可能通过调节IL-6发挥免疫调节作用。
Objective To observe the global and local effects of umbilical cord mesenchymal stem cells(UC-MSCs)on psoriasis-like lesions in mice induced by imiquimod(IMQ).Methods C57 BL/6 mice were randomly divided into control group,model group and hUC-MSCs group.Umbilical cord mesenchymal stem cells of 2×10^(6)were sc before and 6 days after IMQ administration,and saline was sc to mice in control and model group.The skin lesions of psoriasis-like mice were observed by PASI score;the thickness of epidermis,the degree of keratinization and the infiltration of inflammatory cells were observed by HE staining;venous blood of mice was collected to detect the concentration of IL-6 to evaluate the inflammatory reaction of mice.Results Compared with the model group of the same period,the skin lesion symptom of hUC-MSCs injection group was lighter,the PASI score was lower than that of the model group at the same time.The number of dyskeratosis cells was significantly reduced,the degree of erythrocytic overflow,dermal hyperplasia and inflammatory cell infiltration in hUC-MSCs injection group was significantly lower than that in model group.The thickness of epidermis in the model group was significantly lower than that in the model group 6 days after modeling(P<0.01).IL-6 concentration was significantly lower than that in model group on the 12 th day after IMQ treatment.Conclusion hUC-MSCs can delay the occurrence of psoriasis,relieve the symptoms of psoriasis and accelerate the recovery of psoriasis like lesions in psoriasis like mice,it may play an immunomodulatory role by regulating IL-6.
作者
李政楠
王秀娟
李伟
牟春琳
杨莹
金星
徐永胜
LI Zhengnan;WANG Xiujuan;LI Wei;MOU Chunlin;YANG Ying;JIN Xing;XU Yongsheng(Ever Union Biotechnology Company Limited,Tianjin 301925,China)
出处
《药物评价研究》
CAS
2020年第12期2375-2379,共5页
Drug Evaluation Research
基金
国家重点研发计划“干细胞及转化研究”重点专项(2019YFA0112100)
天津市科技计划项目(17ZXXYSY00020)。
