摘要
目的采用超高效液相色谱(UPLC)法建立辛夷花Magnolia biondii药材指纹图谱,并同时测定3种有效成分的含量,评价不同产地辛夷药材质量的差异。方法采用UPLC法进行测定,色谱柱为YMC Triart C_(18)(100 mm×2.1 mm,1.9μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱,体积流量为0.3m L/min,波长为230nm,柱温为30℃,进样量为1μL;建立15批辛夷药材指纹图谱,通过对照品比对,对共有峰进行指认,并对3种有效成分的含量进行测定;对15批辛夷药材指纹图谱进行相似度评价、聚类分析(HCA)和主成分分析(PCA),并利用正交偏最小二乘法-判别分析(OPLSDA)寻找不同产地辛夷药材的差异性成分。结果辛夷药材指纹图谱有10个共有峰,指认出其中3个峰,分别为松脂素二甲醚、木兰脂素和木兰花碱;湖北、四川与河南产区药材的相似度为0.585~0.793;HCA和PCA分析将15批辛夷药材划分为3类,分别对应3个不同的产区,OPLS-DA法确定7个差异标志物,差异显著性排序为木兰脂素>峰4>松脂素二甲醚>峰3>峰8>峰9>峰10。河南产区辛夷木兰脂素和松脂素二甲醚含量最高,湖北产区辛夷木兰花碱含量最高。结论该方法能有效地分析不同产地辛夷药材质量的差异性,为不同产地辛夷药材质量的评价提供参考。
Objective To evaluate the difference of Magnoliae Flos from different producing areas by establishing UPLC fingerprint and determining contents of three effective components of Magnoliae Flos.Methods UPLC method was adopted.The determination was performed on YMC Triart C_(18)column(100 mm×2.1 mm,1.9μm)with acetonitrile-0.1%phosphoric acid solution as the mobile phase by gradient elution at a flow rate of 0.3 mL/min.The detective wavelength was 230 nm,the column temperature was 30℃,the injection volume was 1μL.The UPLC fingerprints of 15 batches of Magnoliae Flos were established and the common peaks were identificated by reference substances.The contents of three effective components were determined.Similarity evaluation,cluster analysis(HCA),and principal component analysis(PCA)were carried out on the fingerprints and orthogonal partial least squares discriminant analysis(OPLS-DA)was used to find the different components of Magnoliae Flos from different producing areas.Results There were 10 common peaks in the fingerprints of Magnoliae Flos.By comparing with the reference substances,three common peaks were identified,namely,magnolin,pinoresinol dimethyl ether,and magnolflorine.The similarity among Hubei,Sichuan and Henan was 0.585—0.793.According to the analysis of HCA and PCA,15 batches of Magnoliae Flos were divided into three categories,corresponding to three different producing areas.Seven kinds of biomarkers were determined by OPLS-DA method.The order of significance was magnolin>peak 4>pinoresinol dimethyl ether>peak 3>peak 8>peak 9>peak 10,respectively.The contents of magnolin and pinoresinol dimethyl ether in Magnoliae Flos of Henan Province were the highest and the content of magnolflorine in Magnoliae Flos of Hubei Province was the highest.Conclusion This method can effectively analyze the quality differences of Magnoliae Flos from different producing areas and provide reference for the quality evaluation.
作者
李振雨
何嘉莹
童培珍
魏梅
孙冬梅
邓淙友
陈向东
LI Zhen-yu;HE Jia-ying;TONG Pei-zhen;WEI Mei;SUN Dong-mei;DENG Cong-you;CHEN Xiang-dong(Guangdong Key Laboratory of Traditional Chinese Medicine FormulaGranule,GuangdongYifong Pharmaceutical Co.,Ltd.,Foshan 528244,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第1期234-240,共7页
Chinese Traditional and Herbal Drugs
基金
广东省省级科技计划项目(2018B030323004)。
关键词
辛夷
UPLC指纹图谱
多成分定量
差异标志物
木兰脂素
松脂素二甲醚
Magnoliae Flos
UPLC fingerprints
multi-components determination
differential marker
magnolin
pinoresinol dimethyl ether
