摘要
核酸检测作为新型冠状病毒肺炎(COVID-19)筛查诊断和病情监测的主要手段,在疫情防控中发挥了重要作用。虽然实时荧光定量PCR被认为是新型冠状病毒(SARS-CoV-2)核酸检测的金标准,但其依赖荧光定量PCR仪且扩增检测时间较长,难以实现现场快速检测。因此许多基于核酸等温扩增的SARS-CoV-2检测方法相继诞生。等温扩增对仪器温控要求不高,通过与微流控芯片和可视化检测技术结合,可进一步简化操作、降低成本,为SARS-CoV-2现场快速筛查提供有力的技术支撑。本文围绕已报道的SARS-CoV-2等温扩增检测方法原理、检测性能及优缺点进行探讨,为进一步发展SARS-CoV-2现场快速检测平台提供参考。
Detection of nucleic acids is the main method for detection and monitoring of SARS-CoV-2,and has played an important part in the prevention and control of the disease caused by SARS-CoV-2 infection:coronavirus disease 2019(COVID-19).Real-time reverse transcription-quantitative polymerase chain reaction(RT-qPCR)is regarded as the"gold standard"for SARS-CoV-2 detection.However,RT-qPCR requires instrumentation and a long detection time,which hampers rapid point-of-care detection.Therefore,several detection methods for SARS-CoV-2 based on isothermal amplification have been developed.Different from RTqPCR,isothermal amplification does not need a thermal cycler for temperature control.Moreover,it can further simplify the operation and reduce costs by combining with technology based on microfluidic chips and visual detection.Therefore,it is an"ideal"technology for rapid detection of SARS-CoV-2 at the point of care.Here,we reviewed the principles,detection performances,advantages and disadvantages of currently reported isothermal-amplification methods for SARS-CoV-2 detection.We have also provided a reference for further development of a rapid detection platform for SARS-CoV-2.
作者
李博
邹秉杰
马雪萍
武海萍
张晏洁
周国华
LI Bo;ZOU Bingjie;MA Xueping;WU Haiping;ZHANG Yanjie;ZHOU Guohua(School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China;Department of Clinical Pharmacy,General Hospital of Eastern Military Command,Nanjing 210002,China;School of Pharmaceutical Sciences,Southern Medical University,Guangzhou,China)
出处
《病毒学报》
CAS
CSCD
北大核心
2021年第1期191-200,共10页
Chinese Journal of Virology
基金
国家自然科学基金(项目号:61871403),题目:基于核酸信号扩增的基因突变数字化检测新方法研究
国家自然科学基金(项目号:81703474),题目:基于核酸扩增的胰腺癌相关磷脂酰肌醇聚糖-1外泌体检测新方法研究与临床应用评价
江苏省杰出青年基金项目(项目号:BK20180005),题目:基于结构识别核酸内切酶的基因检测与基因编辑新方法。
