摘要
目的:通过检测宫颈癌组织中高尔基体鳞酸化蛋白3(GOLPH3)与血管内皮生长因子(VEGF)表达水平,探讨二者在宫颈癌组织中浸润、转移的相互关系。方法:通过免疫组织化学SP法检测43例手术切除的宫颈癌组织及宫颈癌旁组织中GOLPH3和VEGF蛋白表达情况。GOLPH3与VEGF在宫颈癌组织表达的相关性用Pearson相关性分析。结果:GOLPH3和VEGF在宫颈癌组织中的阳性表达率为72.1%、74.42%,显著高于宫颈癌旁组织的37.21%、32.56%(P<0.01)。GOLPH3、VEGF在宫颈癌组织中的表达与病人的肿瘤大小、分化程度、手术病理分期、淋巴结转移有显著相关性(P<0.05~P<0.01),与病人年龄、病理类型无明显相关性(P>0.05)。免疫组织化学结果显示,在GOLPH3阳性表达组的宫颈癌组织中VEGF阳性表达率为83.87%,明显高于GOLPH3阴性表达组的33.33%(P<0.01),宫颈癌组织中GOLPH3表达与VEGF的表达呈正相关性(r=0.494,P<0.01)。结论:GOLPH3过表达通过上调VEGF的表达促进宫颈癌的癌组织中血管生成,从而促进了宫颈癌的浸润和转移。
Objective:To detect the expression levels of Golgi phosphoprotein 3(GOLPH3)and vascular endothelial growth factor(VEGF)in cervical cancer tissues,and explore their relationship in invasion and metastasis of cervical cancer.Methods:The expression levels of GOLPH3 and VEGF proteins in cervical carcinoma tissues and para-cervical cancer tissues of 43 patients were detected using immunohistochemical SP method.The correlations of GOLPH3 and VEGF levels in cervical cancer tissues were analyzed using Pearson correlation analysis.Results:The positive expression rates of GOLPH3 and VEGF in cervicer cancer tissues were 72.1%and 74.42%,respectively,which were significantly higher than those in para-cervical cancer tissues(37.21%and 32.56%)(P<0.01).The expression levels of GOLPH3 and VEGF in cervical cancer tissue were significantly correlated with the tumor size,differentiation,surgical and pathological stage,and lymph node metastasis(P<0.05 to P<0.01),and not significantly correlated with the age and pathological type of patients(P>0.05).The results of immunohistochemistry showed that the positive expression rate of VEGF in cervical cancer tissues with positive GOLPH3 expression group(83.87%)was significantly higher than that in negative GOLPH3 expression group(33.33%)(P<0.01).The expression rate of GOLPH3 was positively correlated with VEGF expression in cervical cancer tissues(r=0.494,P<0.01).Conclusions:The overexpression of GOLPH3 promotes the angiogenesis in cervical cancer tissues by upregulating the expression of VEGF,which leads to the invasion and metastasis of cervical cancer.
作者
苏文芳
SU Wen-fang(Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233040,China)
出处
《蚌埠医学院学报》
CAS
2021年第2期169-172,共4页
Journal of Bengbu Medical College
基金
蚌埠医学院科技发展基金(BYKF17110)。
