摘要
基于超高效液相色谱-三重四级杆串联质谱(UPLC-QqQ-MS/MS)联用技术对茶叶中黄酮醇糖苷(FGs)种类及含量进行测定并对提取方式和检测条件进行优化。用75%(体积分数)甲醇水溶液提取目标化合物,再用Waters Acquity HSS T3色谱柱(粒径为1.8μm,长度×内径为100.0 mm×2.1 mm)分离目标化合物,以含有0.1%(体积分数)甲酸的乙腈溶液为流动相进行梯度洗脱,采用电喷雾正离子源(ESI+)与质谱多反应监测(MRM)方法对成品茶中15种FGs进行定量测定。结果表明,在0.1~20.0μg/ml的质量浓度范围内FGs的基质标准曲线的线性关系良好,其检测限(LOD)为0.08~0.23μg/L,定量限(LOQ)为0.25~0.76μg/L,相对标准偏差(RSD)为1.30%~2.80%,出峰时间为3.60~7.40 min。与现有最优检测技术相比,超高效液相色谱-三重四级杆串联质谱法具有分析速度快、灵敏度高、稳定性好等特点,可为提高茶叶品质成分的检测效率提供参考。
The extraction methods and detection conditions of flavonol glycosides(FGs)in teas were optimized based on ultra-high performance liquid chromatography combined with triple four-bar tandem mass spectrometry(UPLC-QqQ-MS/MS)technology,and the varieties and contents were determined.The target compounds were extracted with 75%(volume fraction)methanol aqueous solution,separated on Waters Acquity HSS T3 column(particle size was 1.8μm,length×inner diameter was 100.0 mm×2.1 mm),and gradient elution was carried out using acetonitrile solution containing 0.1%(volume fraction)formic acid as moving phase.Positive electrospray ionization(ESI+)and mass spectrometry multiple reaction monitoring(MRM)methods were used to quantitatively determine 15 flavonol glycosides in made teas.The results indicated that the matrix standard curve of FGs showed a good linearity in the concentration range of 0.1-20.0μg/ml,the limit of detection(LOD)was 0.08-0.23μg/L,the limit of quantity(LOQ)was 0.25-0.76μg/L,the relative standard deviation(RSD)was 1.30%-2.80%,and the peak out time was 3.60-7.40 min.Compared with the existing optimal detection technology,UPLC-QqQ-MS/MS method has fast analysis speed,high sensitivity and good stability,which can provide reference for improving the detection efficiency of tea quality components.
作者
董方
张群峰
阮建云
DONG Fang;ZHANG Qun-feng;RUAN Jian-yun(Institute of Horticulture, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China)
出处
《江苏农业学报》
CSCD
北大核心
2021年第1期204-212,共9页
Jiangsu Journal of Agricultural Sciences
基金
国家重点研发计划项目(2016YFD0200900)
国家茶产业技术体系建设专项(CARS-19)。
关键词
茶叶
黄酮醇糖苷
超高效液相色谱-三重四级杆串联质谱法
质谱多反应监测
优化
tea
flavonol glycosides
ultra-high performance liquid chromatography combined with triple four-bar tandem mass spectrometry
mass spectrometry multiple reaction monitoring
optimization
